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Pleiotropic gene associated protein from wheat, encoding gene thereof and application

A technology for encoding genes and genes, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as ineffective application of wheat breeding and production

Inactive Publication Date: 2014-04-16
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, a total of 21 wheat stalk-reducing genes have been identified and numbered uniformly, but the dwarf genes widely used and promoted in production are only Rht1, Rht2, Rht8 and Rht9, and most of the other dwarf genes are not effective. application in wheat breeding

Method used

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  • Pleiotropic gene associated protein from wheat, encoding gene thereof and application
  • Pleiotropic gene associated protein from wheat, encoding gene thereof and application
  • Pleiotropic gene associated protein from wheat, encoding gene thereof and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1, construction and phenotype analysis of Rht-Blc near-isogenic line

[0061] First, we used Yanzhan No.1 (Henan local variety, hereinafter referred to as NIL-Rht-Bla) as the recurrent parent, and Thumbiao as the donor parent to backcross for 7 generations to construct the near isogenic gene of the dwarf gene Rht-Blc line (expressed as NIL-Rht-Blc), and then planted in the long-day region-Hebei, and the short-day region-Yunnan, respectively, to observe the field phenotype, investigate its plant height, tiller angle, heading date, panicle germination , grain plumpness and other traits ( figure 1 , A is NIL-Rht-Bla, B is NIL-Rht-Blc). Field survey results show that the average plant height of the constructed dwarf gene near isogenic line NIL-Rht-Blc is only 30 cm, which is significantly lower than the plant height of NIL-Rht-Bla ( figure 1 a, c). There are also obvious differences in the grains observed after harvesting. The grains of NIL-Rht-Blc are relativ...

Embodiment 2

[0064] Embodiment 2, the acquisition of wheat protein Rht-Blc and its coding gene Rht-Blc

[0065] 1. Acquisition of Rht-Blc Genomic DNA Sequence in Wheat

[0066] First design primers spanning the start codon and stop codon to amplify the full length of the gene: Rht-Blcp 5'-ATGCCG TCT ACA ACT ACT ACG CTG-3' and 5'-AGT CCG GCC CGT GCT TAT TTT G-3' . Using Chinese spring (Rht-Bla), Nonglin 10 (Rht-Blb) and thumb dwarf (Rht-Blc) genomic DNA as templates, PCR amplification was carried out with primer Rht-Blcp, and the PCR products were carried out by agarose gel electrophoresis Detection, the detection results showed that the expected 2Kb amplified fragment was obtained in Zhongguochun and Nonglin 10, and a new amplified fragment was obtained in Thumbiao which was about 2Kb significantly larger than that of Zhongguochun and Nonglin 10 ( figure 2 Middle a). The fragment was cloned and sequenced to obtain the genomic DNA sequence of the Rht-Blc gene in the thumb dwarf, which c...

Embodiment 3

[0078] Verification of embodiment 3Rht-Blc gene function

[0079] In order to verify the correctness of the gene sequence we obtained, we designed primers Rht-BlcM ​​(5'-ATG CCG TCT ACA ACT ACT ACG CTG-3'5' -TAG TGC ACG GTG TCC GTG GCG A-3'), this primer can be directly used for specific detection of Rht-Blc gene. When the Rht-Blc gene is contained, the amplified fragment is 2515bp, otherwise the amplified fragment is 489bp. Here we used the primer Rht-BlcM ​​to detect the near-isogenic line of the dwarf gene constructed in Example 1 (represented by NIL-Rht-Blc), and the F2 segregation population of RIL-Rht-Blc. The detection results showed that all dwarf plants contained the Rht-Blc gene, while the Rht-Blc gene could not be detected in the tall stem materials ( image 3 Middle a, Rht-Blc is a dwarf plant, Rht-bla is a tall plant). This detection result indicated that Rht-Blc gene was directly related to plant height, panicle germination, tiller angle, heading date and seed...

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Abstract

The invention discloses a pleiotropic gene associated protein from wheat, an encoding gene thereof and an application. The pleiotropic gene associated protein from wheat is a protein as the following a) or b): a) a protein formed by amino acid sequence as shown in Sequence 3 in a sequence table; and b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence as shown in the Sequence 3 in the sequence table, is derived from (a) and is correlated with any of the following properties: plant height, preharvest sprouting, plant type, heading date and fecundity of wheat. The encoding gene of the protein influences plant height, preharvest sprouting, plant type, fecundity and heading date of wheat. As the gene has strong pleiotropic effects, the gen is of great significance in wheat breeding and production.

Description

technical field [0001] The invention relates to a pleiotropic gene-related protein derived from wheat, its coding gene and application. Background technique [0002] The effect of dwarf and semi-dwarf plants not only improves the crop's fertilizer tolerance and lodging resistance, but more importantly, it reduces the assimilation of stems and transfers more photosynthetic products to the ears, thereby improving the harvest index. Significantly increased crop yields. The agricultural green revolution that occurred in the 1950s and 1960s was due to the widespread promotion of wheat and rice semi-dwarf varieties and the adoption of corresponding cultivation techniques, which greatly increased the yield of wheat and rice. So far, a total of 21 wheat stalk-reducing genes have been identified and numbered uniformly. However, only Rht1, Rht2, Rht8 and Rht9 are widely used and promoted in production, and most of the other dwarf genes are not effective. application in wheat breedin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/11C12N15/113C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N7/01C12Q1/68A01H5/00
Inventor 贾继增武晶孔秀英
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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