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Urease-producing microorganisms and method for solidifying heavy metals in foundation using same

A microbial and urease-producing technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of incomplete removal, high cost, and generation of toxic sludge or other wastes, achieving good results and low cost Low, short-term effect

Active Publication Date: 2012-10-03
TSINGHUA UNIV
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Problems solved by technology

[0003] Traditional heavy metal treatment methods mainly include: chemical precipitation method, ion exchange method, evaporation concentration method, electrolysis method, activated carbon and silica gel adsorption method and membrane separation method, etc., but these methods have incomplete removal, high cost, generation of toxic sludge or Disadvantages such as other waste

Method used

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  • Urease-producing microorganisms and method for solidifying heavy metals in foundation using same
  • Urease-producing microorganisms and method for solidifying heavy metals in foundation using same
  • Urease-producing microorganisms and method for solidifying heavy metals in foundation using same

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Embodiment

[0032] Isolation and Screening of Urease-Producing Bacteria with the Ability to Immobilize Heavy Metals

[0033] 1. Sample collection

[0034] Sporosarcina pasteurii was obtained from the American type culture collection (American type culture collection), numbered ATCC11859, and Terrabacter tumescens was obtained from the General Microbiology Center of China Committee for the Collection of Microbial Cultures, numbered AS.1.2690.

[0035] 2. Isolation and screening of urease-producing strains

[0036] Urease-producing strains were isolated from soil samples collected from the Tsinghua University flower garden.

[0037] Bacteria have ureolytic enzymes, which can decompose urea to produce a large amount of ammonia, making the medium alkaline and red. In this experiment, this characteristic was used to enrich and culture the test samples under the conditions of 37°C and 5M high-concentration urea for 24 hours, and kill various microbial vegetative cells that could not tolerate ...

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Abstract

Urease-producing microorganisms of Sporosarcina antarctica UR53, Sporosarcina koreensis UR47, Sporosarcina sp. UR31 and Bacillus lentus UR41 were preserved in China General Microbiological Culture Collection Center (CGMCC) on March 16, 2012 with the preservation numbers of CGMCC NO.5916, CGMCC NO.5915, CGMCC NO.5913 and CGMCC NO.5914, respectively. The method for solidifying heavy metals in the foundation by using the urease-producing microorganisms comprises the steps of: carrying out fermentation culture on the four microorganisms and microorganisms of Sporosarcina pasteuril and Terrabacter tumescens in a fermentation culture medium to obtain microorganism liquid, adding reaction liquid urea to the microorganism liquid, and then adding to the solution containing heavy metals to form a mixed solution, so that microorganism-heavy metal flocculates are formed in the mixed solution and then water-insoluble heavy metal carbonates are further formed. The method for solidifying the heavy metals in the foundation by using the microorganisms has the advantages of short solidifying time, good effect, low cost and no secondary pollution to environment.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and solidifying heavy metals thereof, in particular to urease-producing microorganisms and a method for solidifying heavy metals in foundations. Background technique [0002] Heavy metal pollution means that due to human production and activities, the content of heavy metals in the environment is significantly higher than its background value. Due to the long-term retention and non-degradable characteristics of heavy metal ions, it has caused great damage to the ecological environment. With large-scale urban expansion and construction, many buildings are built near abandoned factories and garbage dumps, and the heavy metal pollution in the foundation is serious. Heavy metal elements can finally enter the organism through the food chain, destroy the normal metabolism of the organism, and seriously endanger human health. It has become an environmental problem that cannot be ignored. [0003]...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01C12R1/07
Inventor 程晓辉李萌郭红仙
Owner TSINGHUA UNIV
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