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Method for screening functional flora

A functional bacteria and function technology, applied in biochemical equipment and methods, microbial determination/inspection, microorganisms, etc., can solve the problems of high fermentation conditions, reduced function of single bacteria, and narrow adaptation range, and achieve broad application prospects, Stable composition and low cost effect

Inactive Publication Date: 2012-10-03
CHINA AGRI UNIV
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Problems solved by technology

However, the above two bacterial screening methods have deficiencies, which affect their practical application value.
During the purification process of a single bacterium, on the one hand, it loses the opportunity to coexist with other bacteria that have been in a long-term synergistic relationship under natural conditions, resulting in a greatly reduced function of the single bacterium; on the other hand, the purification of a single bacterium makes it suitable for growth conditions More and more narrow, high requirements for fermentation conditions, high fermentation cost; moreover, a single bacterium grows in isolation and is easily polluted and inhibited by other bacteria, making it difficult to grow and function
The strains contained in the natural fermented bacterial agent are multi-bacteria associations assembled disorderly in the natural passive fermentation process. It is impossible to grasp the composition of the strains, lack of science, and the use effect is difficult to control; and natural fermentation The fermentation process of bacterial agents follows the law of passive natural circulation, its decomposition efficiency is low, and the effect is unstable

Method used

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  • Method for screening functional flora

Examples

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Embodiment 1

[0025] Embodiment 1, screening the compound flora of decomposing lignocellulose

[0026] 1. Screening of complex flora

[0027] 1. Bacterial source

[0028] The rotten bottom of the straw pile was used as the fungus source.

[0029] Because the main component of the straw in the straw woodpile is lignocellulose, the rot of the woodpile indicates that the straw is rotten, which in turn indicates that the lignocellulose is degraded, and the degradation of lignocellulose must be caused by the flora that decomposes lignocellulose. From this, it can be concluded that the rotten straw must contain lignocellulose-decomposing flora, and the rotten pile bottom must contain lignocellulose-decomposing flora, so the rotten pile bottom is used as the bacterial source; The habitat matrix of the flora, which contains rotten straw, soil, flora that decomposes lignocellulose, etc.

[0030] 2. Restricted culture and screening

[0031] Experimental group: Inoculate 5.0g of bacterial source s...

Embodiment 2

[0065] Example 2. Screening of complex flora for preparing straw fermented feed

[0066] 1. Screening of complex flora

[0067] 1. Bacterial source

[0068] Straw from rice-producing areas in Northeast China was used as the bacterial source.

[0069] 2. Restricted culture and screening of complex flora

[0070] Experimental group: Take 0.5g of bacterial source straw and insert it into a conical flask containing 10ml of restrictive medium, culture it anaerobically in a refrigerator at 5°C, and cultivate it for 10 days (denoted as the 0th generation culture), and obtain a culture (the triangular flask All the substances in it are called culture together); then take 15 μl of the culture and inoculate it into fresh restrictive medium, culture it anaerobically at 5°C for 4 days (referred to as the first generation culture); then transfer the culture into fresh The limited medium was cultured under the same conditions as the first generation culture, and successively subcultured ...

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Abstract

The invention discloses a method for screening a functional flora. The method comprises the following steps of: 1) selecting a substrate containing a functional flora; 2) inoculating the substrate in a limiting culture medium, culturing and performing subculture; 3) establishing indexes for evaluating the functions of the functional flora, and detecting the indexes and detecting whether a strain composition in the functional flora is stable in the process of the culture of each generation; and 4) thus obtaining the functional flora in the process of the culture of a certain generation if the indexes meet the functional requirements of the functional flora and the strain composition is stable in the process of the culture of the certain generation. The functional flora obtained by the method can continue to keep a synergistic effect among all kinds of bacteria, and has a stable composition and powerful functions, and the action force cannot be weakened because the functional flora is separated from the natural world; and in addition, the method is easy to operate, low in cost, time-saving and labor-saving. Therefore, the method has a broad application prospect in the field of the screening of functional bacteria.

Description

technical field [0001] The invention relates to a method for screening functional flora. Background technique [0002] At present, there are generally two types of screening methods for strains: the purification and screening of single bacteria and the screening and preparation of natural fermented bacterial agents. The purification and screening of a single bacterium is to separate and purify from nature according to the required functional requirements to obtain a single bacterium of interest; the screening of natural fermentation product bacterial agents is to mix and accumulate natural materials in a certain proportion, so that they can be slowly fermented under natural conditions to obtain Natural fermentation agent. However, the above two bacterial screening methods have deficiencies, which affect their practical application value. During the purification process of a single bacterium, on the one hand, it loses the opportunity to coexist with other bacteria that have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12Q1/68C12Q1/04
Inventor 崔宗均王小芬
Owner CHINA AGRI UNIV
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