Plant root layered membrane-forming enhanced water body repairing method for biological denitrification
A plant root system and biological denitrification technology, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve problems such as affecting hydraulic flow, heavy floating bed load, floating bed sinking, etc. Achieve the effects of promoting anaerobic denitrification, good biocompatibility, and promoting nitrification
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[0015] 1. Enrichment of denitrifying bacteria
[0016] The heavily polluted river sediment was inoculated into the denitrifying bacteria culture medium, and acclimatized at a constant temperature of 28°C for 20 days under anoxic conditions, and the obtained enriched bacterial liquid was centrifuged at 2000 r / min for 10 min, and then centrifuged with 0.9% w / v saline Wash 2-3 times, then dilute with 0.9% w / v normal saline according to the volume ratio of bacteria to normal saline at 5:1, mix well, prepare concentrated bacterial suspension, and store at 4°C for later use. The culture medium for denitrifying bacteria is as follows: add 5 g of sodium citrate, 2 g of potassium nitrate, 1 g of dipotassium hydrogen phosphate, 1 g of potassium dihydrogen phosphate, and 0.2 g of magnesium sulfate heptahydrate in every liter of distilled water, adjust the pH to 7.2, mix After homogeneity, sterilize under high temperature and high pressure at 121°C for 20 minutes.
[0017] 2. Enrichment ...
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