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Whole-time three-wavelength fusion method for simultaneously determining contents of four ingredients in Flos Carthami

A full-time, three-wavelength technology, applied in measurement devices, material separation, analysis of materials, etc., can solve the problems of uneven quality of safflower medicinal materials, and difficulty in ensuring the stability of safflower medicinal materials, and achieve the standardization and guarantee of the process. Balanced, stable, and quality-assured effects

Active Publication Date: 2012-09-26
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

At present, the quality of commercially available safflower medicinal materials is uneven, so the stability of safflower medicinal materials is difficult to guarantee

Method used

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  • Whole-time three-wavelength fusion method for simultaneously determining contents of four ingredients in Flos Carthami
  • Whole-time three-wavelength fusion method for simultaneously determining contents of four ingredients in Flos Carthami
  • Whole-time three-wavelength fusion method for simultaneously determining contents of four ingredients in Flos Carthami

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Embodiment Construction

[0035] The method for simultaneously determining the content of four components in the traditional Chinese medicine safflower by three-wavelength fusion at the same time is characterized in that it is carried out according to the following steps:

[0036] 1. Preparation of the test solution: take 2 g of the safflower medicinal material, accurately weigh it, put it in a conical flask, add 50 mL of methanol precisely, weigh it, treat it ultrasonically for 40 minutes, let it cool, weigh it again, and use methanol to make up the reduced amount. Shake well, filter, evaporate to dryness, add methanol to dissolve the residue and transfer it to a 10mL volumetric flask, add methanol to the volume, shake well, filter, and get the filtrate.

[0037] The method for controlling the contents of the four components of the safflower medicinal material of the present invention, the preferred detection method: Agilent TC-C18 (4.6mm × 250mm, 5 μm) chromatographic column with octadecyl bonded sili...

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Abstract

The invention discloses a whole-time three-wavelength fusion method for simultaneously determining contents of four ingredients in Flos Carthami. The method combines modern analysis detection means and information processing means, and effectively fuses spectral information of index ingredients of Chinese medicinal materials. The detected ingredients all have maximum ultraviolet absorption wavelength, to improve signal-to-noise ratio of detected ingredients. The method can overcome defect of insufficient information amount in single-wavelength single-index detection, to perfectly reflect inherent quality of Chinese medicinal materials. The inventive quality control method of four ingredients is simple in operation, high in stability and reliability, and good in repeatability; and can be used in quality evaluation and control of Chinese medicinal materials, and research and development of new Chinese medicines; and especially can be used as one index for quality control and authentication of Flos Carthami.

Description

field of invention [0001] The invention belongs to the technical field of detecting the quality of Chinese herbal medicines by means of modern analysis and detection means and information processing means, and relates to a content control method of multi-index components of Chinese herbal medicines, more specifically, a full-time three-wavelength fusion simultaneous measurement Method for the content of four components of traditional Chinese medicine safflower. Background technique [0002] According to the 2010 edition of the Pharmacopoeia of the People's Republic of China, this product is the safflower of Compositae Carthamus tinctorius L. dry flowers. Pick when the flowers turn from yellow to red in summer, and dry in the shade or in the sun. Safflower is one of the precious medicinal materials in China, which was first recorded in "Kaibao Materia Medica", and has the title of "king of blood circulation" in traditional Chinese medicine. This product is pungent, dissipa...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 孟宪生包永睿姚东王帅崔亚玲
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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