PRV, PCV-2, PPV multiple PCR (Polymerase Chain Reaction) detection kit

Abstract

The invention discloses a PRV, PCV-2, PPV multiple PCR (Polymerase Chain Reaction) detection kit which comprises 40 mu L of isopyknic mixed PRV primer, PCV-2 primer and PPV primer, wherein the concentration of the PRV primer, the PCV-2 primer and the PPV primer is 10muM, buffer solution is 600 mu L, negative control is 20 mu L, PCR enzyme is 250 mu L, ultrapure water is 170 mu L, Market DL 2000 is 50 mu L, and positive control is 20 mu L. The invention aims to a condition that the PRV,the PCV-2 and the PPV exist in a same reaction system, designs three types of primers, and adopts the kit made from the three types of primers. By utilizing, the PRV,the PCV-2 and the PPV can be simultaneously detected, the specificity and sensitivity are high, the detection time is short, the detection cost is low, and a scientific basis is provided for prevention and control of pig infectious diseases.

Description

technical field [0001] The invention relates to a reagent kit, in particular to a PRV, PCV-2 and PPV multiplex PCR detection reagent kit. Background technique [0002] With the continuous development of large-scale pig farming, the current reproductive obstacles caused by porcine pseudorabies virus (PRV), porcine circovirus (PCV-2) and porcine parvovirus (PPV) are relatively serious, and mixed infections often occur. The pig industry has caused huge economic losses. The mixed infection of PRV, PCV-2 and PPV is difficult to make a differential diagnosis based on clinical symptoms alone, and the large-scale application of PRV gene deletion vaccines makes it necessary to distinguish between wild virus infection and vaccination. At present, the main detection methods include virus isolation and identification, agar diffusion test, trace serum neutralization test, ELISA, and PCR methods. For PRV gene deletion vaccines, gE-ELISA is used to distinguish wild virus infection from va...

AI Technical Summary

Problems solved by technology

However, it is difficult for conventional diagnostic methods to distinguish diseases with similar symptoms at the same time, and conventional etiological and serological detection methods have the disadvantages of complicated operations, time-consuming and laborious, and low sensitivity.

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