Method for extracting and preparing silymarin from polygonum capitatum herb

A technology of silymarin and Polygonum cephalum, which is applied in the field of medicine, can solve problems such as unexplained extraction and preparation methods of silymarin, and achieve the effects of easy promotion, high yield and low cost

Inactive Publication Date: 2012-09-19
NO 97 HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The inventor's master's degree thesis [Yang Yang. Study on the chemical constituents of Sage ganxie and Polygonum cephalum. Shanghai: Second Military Medical University, 2009] reported for the first time that Polygonum cephalosa contained flavonoid lignin compounds such as silybin, but did not clarify the specific extraction and preparation method of silymarin

Method used

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  • Method for extracting and preparing silymarin from polygonum capitatum herb
  • Method for extracting and preparing silymarin from polygonum capitatum herb

Examples

Experimental program
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Effect test

Embodiment 1

[0018] 2000g of dried whole herb of Polygonum cephalosporin, cut into sections, crushed and sieved, the coarse powder was extracted with 5 times the volume of petroleum ether under reflux for 3 times, each time for 1 hour, and the petroleum ether extract was filtered off. After the remaining coarse powder is dried, soak for 24 hours with 2 times the volume of ethanol aqueous solution with a concentration of 80% volume concentration, and extract by percolating with 20 times the volume. The percolation extract was concentrated under reduced pressure at 60°C until it had no alcohol smell, and 524 g of Polygonum cephalosporin fluid extract was obtained, with a yield of 26.2%.

[0019] Add 3-4 times the volume of water to suspend the Polygonum cephalum liquid extract prepared above to make the total volume 1000ml, extract with 10 times the volume of ethyl acetate, concentrate the extract at 60°C under reduced pressure to recover the solvent, and evaporate to dryness Afterwards, 21....

Embodiment 2

[0023] 2000g of dried whole herb of Polygonum cephalosporin, cut into sections, crushed and sieved, the coarse powder was extracted with 5 times the volume of petroleum ether under reflux for 3 times, each time for 1 hour, and the petroleum ether extract was filtered off. After the remaining coarse powder is dried, soak in 2 times the volume of ethanol aqueous solution with a concentration of 85% volume concentration for 24 hours, heat and reflux extraction with 5 times the volume for 3 times, each time for 1 hour, combine the extracts and concentrate under reduced pressure at 60°C until there is no alcohol smell. 582g of Polygonum cephalosporin liquid extract was obtained, and the yield was 29.1%.

[0024] Add 3-4 times the volume of water to suspend the Polygonum cephalum liquid extract prepared above to make the total volume 1000ml, extract with 10 times the volume of ethyl acetate, concentrate the extract at 60°C under reduced pressure to recover the solvent, and evaporate ...

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Abstract

The invention discloses a method for extracting and preparing silymarin from polygonum capitatum herb, and belongs to the technical field of medicines.. The method comprises the following steps of: firstly extracting the polygonum capitatum herb with petroleum ether under refluxing by heating, and removing extracting solution; then soaking with high-concentration ethanol solution, percolating or extracting under refluxing by heating, and concentrating under reduced pressure to obtain fluid extract; adding water into the fluid extract for suspension, extracting with ethyl acetate, carrying out concentration and evaporation on the extracting solution under reduced pressure, then adopting silica gel column chromatography, firstly removing impurities by elution of eluent of petroleum ether and ethyl acetate at a ratio being 2:1, then eluting by using eluent of petroleum ether and ethyl acetate at a ratio being 1:1, and carrying out concentration and evaporation on the eluate under reduced pressure to obtain the light-green silymarin powder.. The method disclosed by the invention has the advantages of no toxicity, environment-friendly effect, no pollution to environment, simpleness in operation, low cost, higher yield and purity of the silymarin and easiness in popularization.

Description

technical field [0001] The invention relates to a method for extracting and preparing silymarin from whole Polygonum cephalum herb, belonging to the technical field of medicine. Background technique [0002] Milk thistle ( Silybum marianum (L.) Gaertn.), for Compositae ( Compositae ) Milk thistle ( Silybum ) plant, also known as pheasant, milk thistle, mouse tendon, annual or biennial herb, originally distributed in Europe, the Mediterranean region, North Africa and central Asia, and is introduced and cultivated in my country. Achenes are used as medicine, with a bitter and cool taste, clearing heat and detoxifying, protecting the liver and benefiting the gallbladder. It is mainly used to treat liver and gallbladder diseases. It has a history of more than 2,000 years. Volume 1. Beijing: Science Press, 1987: 161-162. National Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China (Part One). 2005 edition. Beijing: Chemical Industry Press, 2005: 5...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/04
Inventor 杨阳吕建峰朱斌周忠海刘军权
Owner NO 97 HOSPITAL OF PLA
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