In-vitro culture method for tender stem segments of dendrobium nobile

A cultivation method and technology of Dendrobium candidum, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low reproduction rate, easy to carry viruses, unable to meet production seedlings, etc., and achieve fast reproduction speed and reproduction. high rate effect

Inactive Publication Date: 2012-09-12
上海市闵行区农业科学研究所
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Problems solved by technology

[0002] Dendrobium is a high-grade and precious tropical orchid that likes temperature and humidity. Spring Dendrobium is one of the main species. It has strong adaptability, various and gorgeous colors, and the f

Method used

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Embodiment

[0017] A kind of spring dendrobium orchid's tender stem segment is isolated rapid cultivation method, and concrete steps are:

[0018] Step 1: Select freshly drawn twigs with a length of 10 cm, peel off the leaves and leaf sheaths, wash them twice with detergent, soak and disinfect them with 75vol% ethanol solution for 1 minute on the ultra-clean workbench, and then use Soak and sterilize in Jieermin solution for 15 minutes, the volume ratio of Jieermin and water in the described Jieermin solution is 1:50, and finally soak and disinfect with 0.1vol% mercuric chloride solution for 15 minutes, rinse three times with sterile water, and use Blot dry with sterile filter paper;

[0019] The second step: cut the twigs obtained in the first step into 1 cm long stem segments, each stem segment has 1 node, and inoculate them in the first medium to induce axillary buds to germinate and grow; the first medium is cultured in MS Cytokinin, gibberellin, naphthalene acetic acid and banana pu...

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Abstract

The invention provides an in-vitro culture method for tender stem segments of dendrobium nobile. The in-vitro culture method is characterized by comprising the following specific steps of: step 1, selecting newly-sprouted tender branches, peeling off leaves and sheaths, cleaning, disinfecting, washing by sterile water, and absorbing water by sterile filter paper; step 2, cutting the tender branches obtained in the step 1 into stem segments, and inducing axillary buds to germinate and grow; step 3, cutting off the buds obtained in the step 2, and inducing clustered buds to grow; step 4, cutting the clustered buds into single buds, inoculating the single buds on a third culture medium, and then performing rooting culture to obtain rooted test-tube seedlings; and step 5, transplanting the rooted test-tube seedlings in a moss matrix, placing in a plastic plug and then culturing into 'moss plug seedlings', watering enough after transplanting, starting to spraying clean water on leaf surfaces the next day, dressing nutrient solution after half month, transplanting in a flowerpot after more than three months of culture, and then culturing as potted flowers or plastic shed big seedlings. The seedlings of dendrobium nobile obtained by the culture method provided by the invention are fast in propagation speed and high in propagation rate.

Description

technical field [0001] The invention relates to a rapid in vitro culture method for young stems of dendrobium orchids, which utilizes pumped new shoots with a length of about 10 cm to induce clustered buds after cleaning and disinfection, and then obtains complete test-tube seedlings after culture, and belongs to the technical field of dendrobium orchid cultivation methods . Background technique [0002] Dendrobium is a high-grade and precious tropical orchid that likes temperature and humidity. Spring Dendrobium is one of the main species. It has strong adaptability, various and gorgeous colors, and the flowering period lasts for several months. It has high ornamental value. Its seedlings are propagated by cuttings. , low reproductive rate and easy to carry virus, unable to meet the needs of production seedlings. Contents of the invention [0003] The object of the invention is to provide a method for cultivating spring Dendrobium orchid seedlings with fast propagation s...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 汤桂钧蒋建平胡海峰吴永兰吴穷张媁
Owner 上海市闵行区农业科学研究所
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