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Method for detecting influence of attenuation bacillus calmette-guerin to asthma

A live BCG and detection method technology, which is applied in the field of detection of the impact of live attenuated BCG on asthma, can solve the problem of no corresponding detection method for the impact of asthma

Inactive Publication Date: 2012-09-05
SUZHOU HEALTH COLLEGE
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for detecting the influence of live attenuated BCG on asthma, which solves the problem that there is no corresponding detection method for the influence of live attenuated BCG on asthma

Method used

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Experimental program
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Embodiment 1

[0020] 1 Materials and methods

[0021] 1) Preparation of experimental animals and reagents

[0022] Kunming mice, ovalbumin, live attenuated BCG, anti-TLR4 monoclonal antibody, anti-GAPDH monoclonal antibody, HRP-labeled mouse IgG antibody, etc.

[0023] 2) Grouping of experimental animals and preparation of sensitization models

[0024] The experimental animals were randomly divided into three groups, namely, the blank control group, the asthma onset group and the attenuated BCG intervention group, with 10 mice in each group. Asthma-onset group: sensitized by intraperitoneal injection of 0.08% OVA on days 1, 7, and 14, 0.2ml each time, Al(OH) 3 as an immune adjuvant. On the 15th day, the aerosol challenge began; attenuated live BCG intervention group: the establishment of the asthma model was the same as that of the asthma onset group, and intradermal injection of attenuated live BCG was repeated one day before each sensitization and within one hour of the final aerosol c...

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Abstract

The invention discloses a method for detecting influences of attenuation bacillus calmette-guerin to asthma. The method comprises the following steps of: establishing asthma models of animals, inoculating bacillus calmette-guerin many times, and detecting the expression of lung tissue TLR4 (toll-like receptor 4) of the small animals by western blotting. According to the method for detecting influences of attenuationbacillus calmette-guerin to asthma of the invention, the influences of attenuation bacillus calmette-guerin to asthma are as follows: the therapeutic effects of the bacillus calmette-guerin are relative to the enhancement of the TLR4 (toll-like receptor 4) of the lung tissue, so that the attenuation bacillus calmette-guerin can play an important function in treating the asthma.

Description

technical field [0001] The invention relates to the medical field, in particular to a method for detecting the effect of live attenuated BCG on asthma. Background technique [0002] Airway hyperresponsiveness, Ig-E regulation, and specific response genes are present in asthma. Toll-like receptors are proteins found in mammals that have homology with Drosophila, named TLR, and TLR4, which was originally discovered, is currently the most studied. Studies have confirmed that the mycobacterial lipoprotein of the live attenuated BCG can bind to Toll-like receptors on macrophages, promote the production of IL-12 and IFN-γ by macrophages, and inhibit the production of IL-4 and IL-5. So as to correct the Th1 / Th2 subgroup number and (or) functional imbalance in asthma. [0003] At present, the effect of live attenuated BCG on the expression of TLR4 in asthma is still unknown. Contents of the invention [0004] The purpose of the present invention is to provide a method for detec...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/76
Inventor 张雅琴眭建
Owner SUZHOU HEALTH COLLEGE
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