Extraction method, total saponins and use of Kudingcha holly leaves

A technology of Kudingcha holly and its extraction method, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, metabolic diseases, etc., can solve the problems of little or no effect in reducing blood fat, and consumers spend money, etc.

Active Publication Date: 2014-10-08
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many varieties of Kudingcha on the market. According to investigations, the leaves of 22 kinds of plants can be used as Kudingcha. Consumers spend a lot of money with little or no effect in lowering blood lipids

Method used

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  • Extraction method, total saponins and use of Kudingcha holly leaves
  • Extraction method, total saponins and use of Kudingcha holly leaves
  • Extraction method, total saponins and use of Kudingcha holly leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This example is used to illustrate the extraction method of Kudingcha holly leaves provided by the present invention.

[0031] Extraction: Weigh 0.5Kg of Kudingcha holly leaf medicinal material, reflux extraction with 5L and 4L 60% ethanol respectively and filter twice, the reflux time is 0.5 hour and 1 hour respectively, and combine the filtrates;

[0032] Ethanol removal: Distill the extracted filtrate under reduced pressure until it has no alcohol smell, and recover the ethanol. The relative density of the concentrated solution is 1.06-1.08 (room temperature);

[0033]Adsorption: Adsorb the obtained solution on a HPD400A macroporous resin column, the filling capacity of the resin column is 5Kg, the diameter-to-height ratio is 1:4, the sample is loaded repeatedly for 3 times, and the adsorption is static for 30 minutes;

[0034] Washing: first rinse the resin column with 4 column volumes of deionized water until sugar-free; then rinse with 8 column volumes of 20% alka...

Embodiment 2

[0038] This example is used to illustrate the extraction method of Kudingcha holly leaves provided by the present invention.

[0039] Extraction: Weigh 0.5Kg of Kudingcha holly leaf medicinal material, reflux extraction with 4L of 65% ethanol by volume, 3L of 60% ethanol and 3L of 50% ethanol and filter three times, the reflux time is 2 hours and 1 hour respectively and 0.5 hour, the filtrate was combined;

[0040] Ethanol removal: Distill the extracted filtrate under reduced pressure until it has no alcohol smell, and recover the ethanol. The relative density of the concentrated solution is 1.06-1.08 (room temperature);

[0041] Adsorption: Adsorb the obtained solution on an AB-8 macroporous resin column, the filling capacity of the resin column is 3.5Kg, and the ratio of diameter to height is 1:8;

[0042] Washing: first rinse the resin column with 8 column volumes of deionized water until sugar-free; then rinse with 10 column volumes of 30% alkaline ethanol (NaOH, pH=10.5)...

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Abstract

The invention provides a method for extracting kudingcha holly leaves, total saponins of kudingcha holly leaves extracted by the method and uses thereof. The extraction method comprises the following steps: (1) reflux extracting Kudingcha holly leaves with 50-70% ethanol aqueous solution; (2) filtering the extract and removing ethanol therein; (3) obtaining The solution is adsorbed on a macroporous resin column, and then the resin column is eluted with water, 10-30% alkaline ethanol aqueous solution, 10-30% ethanol aqueous solution and 50-70% ethanol aqueous solution with a pH value of 9-11; (4 ) Concentrate and dry the solution obtained by eluting with 50-70% ethanol aqueous solution. The prepared total saponins can be used to prepare drugs for lowering cholesterol, lowering blood fat and resisting atherosclerosis.

Description

technical field [0001] The invention relates to a method for extracting kudingcha holly leaves, total saponins of kudingcha holly leaves extracted by the method and uses thereof. Specifically, the present invention relates to a method for extracting the total saponins of the leaves of Kudingcha holly. The total saponins extracted by the method can be used for lowering cholesterol, lowering blood fat, and resisting atherosclerosis. Background technique [0002] Hyperlipidemia includes high cholesterol and high triglycerides, and the former is far more harmful than the latter. For hyperlipidemia, drug-induced lipid-lowering therapy is the most effective measure to treat hyperlipidemia. At present, there are many effective drugs for lowering triglycerides, but the effective drugs for lowering cholesterol are mainly statins. This type of drug is mainly used to fight arteriosclerosis caused by hypercholesterolemia, and its mechanism of action is to inhibit the synthesis of chol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P3/06A61P9/10A61K127/00
CPCC07H15/256A61K2236/333A61K31/704A61K36/185A61P3/06A61P9/10
Inventor 屠鹏飞张彤梅郑娇马治中姜勇高雅唐丽车彦云
Owner PEKING UNIV
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