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Quality control method for aristolochic acid A in swelling-reducing and pain-alleviating tincture

A quality control method and aristolochic acid technology, applied in the field of medicine, can solve the problems of insufficient detection accuracy and negative interference, and achieve the effect of high sensitivity and satisfying quality control.

Active Publication Date: 2012-07-18
广西壮族自治区花红药业集团股份公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Anti-swelling and pain-relieving tincture is the exclusive product of Huahong Pharmaceutical Co., Ltd. in Guangxi Zhuang Autonomous Region. "Quality Control Method for Aristolochic Acid A in Asarum Medicinal Materials and Xiaozhongzhitong Tincture" discloses a method for detecting aristolochic acid A in Xiaozhongzhitong tincture. , there are problems such as insufficient detection accuracy and negative interference

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Test product: using anti-swelling and pain-relieving tincture, a commercially available product, produced by Huahong Pharmaceutical Co., Ltd. of Guangxi Zhuang Autonomous Region, batch number: 20081205

[0026] The specific operation of the inspection of the swelling and pain-relieving tincture is as follows:

[0027] 1. Chromatographic conditions:

[0028] Chromatographic column: silica gel column bonded with octadecylsilane;

[0029] The mobile phase is composed of: 0.1% ammonium carbonate solution-acetonitrile with a mass percentage of 10% hydrochloric acid solution to adjust the pH to 7, wherein the volume ratio of the solution to acetonitrile is 70:30;

[0030] Flow rate: 0.8ml / min. ;

[0031] Detection wavelength: 240nm.

[0032] 2. Preparation of the reference solution: Accurately weigh an appropriate amount of aristolochic acid A, add methanol to make a solution containing 1 μg of aristolochic acid A per milliliter.

[0033] 3. Preparation of the test solut...

Embodiment 2

[0037] Test product: using anti-swelling and analgesic tincture, a commercially available product, produced by Huahong Pharmaceutical Co., Ltd. of Guangxi Zhuang Autonomous Region, batch number: 20090201

[0038] The specific operation of the inspection of the swelling and pain-relieving tincture is as follows:

[0039] 1. Chromatographic conditions:

[0040] Chromatographic column: silica gel column bonded with octadecylsilane;

[0041] The composition of mobile phase is: be that 10% hydrochloric acid solution is used mass percent to adjust pH to 7 volume percent 0.1% ammonium carbonate solution-acetonitrile, wherein the volume ratio of solution and acetonitrile is 80:20;

[0042] Flow rate: 1ml / min. ;

[0043] Detection wavelength: 250nm.

[0044] 2. Preparation of the reference solution: Accurately weigh an appropriate amount of aristolochic acid A, add methanol to make a solution containing 2 μg of aristolochic acid A per milliliter.

[0045] 3. Preparation of the tes...

Embodiment 3

[0049] Test product: using anti-tumor and pain-relieving tincture, a commercially available product, produced by Huahong Pharmaceutical Co., Ltd. of Guangxi Zhuang Autonomous Region, batch number: 20101101

[0050] The specific operation of the inspection of the swelling and pain-relieving tincture is as follows:

[0051] 1. Chromatographic conditions:

[0052] Chromatographic column: silica gel column bonded with octadecylsilane;

[0053] The mobile phase is composed of: 0.1% ammonium carbonate solution-acetonitrile with a mass percentage of 10% hydrochloric acid solution to adjust the pH to 7, wherein the volume ratio of the solution to acetonitrile is 90:10;

[0054] Flow rate: 1.5ml / min. ;

[0055] Detection wavelength: 260nm.

[0056]2. Preparation of the reference solution: Accurately weigh an appropriate amount of aristolochic acid A, add methanol to make a solution containing 5 μg of aristolochic acid A per milliliter.

[0057] 3. Preparation of the test solution:...

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Abstract

The invention discloses a detection method for a swelling-reducing and pain-alleviating tincture, which is used for controlling the content of aristolochic acid A in the swelling-reducing and pain-alleviating tincture and ensuring that the content of aristolochic acid A in the swelling-reducing and pain-alleviating tincture meets the safety requirement of a product. Due to the adoption of the method provided by the invention, the detection problem of a small amount of aristolochic acid A is solved, and a high-sensitivity, accurate and reliable detection method for aristolochic acid A is established. On the aspect of operation, the method provided by the invention is accurate and reliable, and has high accuracy and very high practical value.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a quality control method for aristolochic acid A in antitumor and pain-relieving tincture. Background technique [0002] Anti-swelling and pain-relieving tincture is the exclusive product of Huahong Pharmaceutical Co., Ltd. in Guangxi Zhuang Autonomous Region. "Quality Control Method for Aristolochic Acid A in Asarum Medicinal Materials and Xiaozhongzhitong Tincture" discloses a method for detecting aristolochic acid A in Xiaozhongzhitong tincture. , there are problems such as insufficient detection accuracy and negative interference. [0003] Therefore, it is necessary to carry out further research on quality control methods to find a quality control method for aristolochic acid A with higher precision, and to carry out quality control of aristolochic acid A in Xiaozhongzhitong tincture. Contents of the invention [0004] The invention provides a method for quality control of the ant...

Claims

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Application Information

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IPC IPC(8): A61K36/9066G01N30/36A61P29/00A61K31/125
Inventor 唐秀玲
Owner 广西壮族自治区花红药业集团股份公司
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