Method for preparing small-molecule collagen polypeptide powder by using poultry skin and application of small-molecule collagen polypeptide powder in fruit juice beverage
A technology of collagen polypeptide and collagen, which is applied in the field of small molecule collagen polypeptide fruit juice drink and its processing, can solve the problem of bitter taste of the beverage, and achieve the effects of easy absorption, strong biological activity, and maintaining skin elasticity
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Embodiment 1
[0039] Example 1: Preparation of small molecule collagen polypeptide from chicken skin and its uniform dispersion method in orange juice drink
[0040] (1) Take 100 kg of chicken skin and clean it up (≤1cm) to obtain clean raw materials, and then add it to a 10% salt solution (material-to-liquid mass ratio 1:10), and stir and extract at room temperature for 12 hours.
[0041] (2) After washing the chicken skin treated according to (1) several times, add water according to the material-to-liquid ratio of 1:10, add 1000 g of lipase and carry out agitation and degreasing treatment for 8 hours at a temperature of 35°C.
[0042] (3) Wash the chicken skin after degreasing treatment several times, add 10% ethanol according to the material-to-liquid ratio of 1:10, adjust the pH to 7, add 800g according to the mass ratio of papain: complex protease 1:1, and hydrolyze at 45°C for 3h , The hydrolyzed solution is inactivated at 85°C and filtered to obtain a molecular weight of 2000-5000 Dalton (...
Embodiment 2
[0045] Example 2: Preparation of small molecule collagen polypeptide from duck skin and its uniform dispersion method in grapefruit juice drink
[0046] (1) Take 100 kg of duck skin and clean it up (≤1cm) to obtain clean raw materials, then add it to a 5% salt solution (material-to-liquid ratio 1:50), and stir and extract at room temperature for 24 hours.
[0047] (2) After washing the duck skin treated according to (1) several times, add water according to the material-to-liquid ratio of 1:50, add 1200g of lipase, and carry out agitation and degreasing treatment for 12h at a temperature of 45°C.
[0048] (3) Clean the fat-removing material several times, add 20% ethanol according to the material-liquid ratio 1:50, adjust the pH to 7.5, add 1000g according to papain: compound protease=2:1, hydrolyze at 50℃ for 3.5h, The hydrolyzed solution is inactivated at 85°C and filtered to obtain a collagen polypeptide solution with a molecular weight of 2000-5000 Dalton.
[0049] (4) Drying and ...
Embodiment 3
[0051] Example 3: Preparation of small molecule collagen polypeptide from goose skin and its uniform dispersion method in apple juice drink
[0052] (1) Take 100 kg of goose skin and wash it into pieces (≤1cm) to obtain clean raw materials, then add it to 8% salt solution (material-to-liquid ratio 1:25), and stir and extract at room temperature for 24 hours.
[0053] (2) After washing the goose skin treated according to (1) several times, add water according to the material-to-liquid ratio of 1:25, and perform agitation and degreasing treatment with 800g of lipase for 12h. The temperature is 45°C.
[0054] (3) Clean the fat-removing material several times, add 30% ethanol according to the material-to-liquid ratio of 1:25, adjust the pH to 6.5, add 900g according to papain: compound protease=1:2, hydrolyze at 55℃ for 4h, and hydrolyze The latter solution is enzyme-inactivated at 85°C and filtered to obtain a collagen polypeptide solution with a molecular weight of 2000-5000 Dalton.
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