Urine cell separation chip and reagent

A technique for urine cells and reagents, applied in the field of urine cell separation chips and reagents, can solve the problems of poor repeatability of urine cells, low quantitative determination ability of formed components, low urine cell discrimination ability, etc.

Inactive Publication Date: 2012-07-11
孙续国 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem solved by the present invention is to overcome the shortcomings of urine flow cytometry chip technology for urine casts, urine cells, low discrimination ability, poor repeatability and high-speed photography technology for quantitative determination of urine cells and other formed elements.

Method used

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  • Urine cell separation chip and reagent
  • Urine cell separation chip and reagent
  • Urine cell separation chip and reagent

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example

[0020] The name given to Li Wensheng et al. on August 3, 2005 was "A Method and Device for Making Cell Chips" Announcement Number: CN1661039. A method for preparing a cell chip and a device for implementing the method are disclosed in detail. Reflected in the cavity structure formed between the array tube and the inner core, the cell sample is embedded in the cavity through agarose, and the cell agarose embedding block is formed between the outer jacket and the bottom fixed plate with a microporous structure In the enlarged cavity, dehydration, transparency, wax immersion, etc. are processed, and then the array tube device is used to form paraffin hollow holes, while gently pushing the cylinder core to make the cell agar-embedded block fall into the array holes to form cells The chip is embedded in paraffin, and then sliced ​​and stained to complete the preparation of the cell chip.

[0021] The announcement number of a microfluidic cell chip authorized on September 24, 2008 at ...

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Abstract

The invention discloses a chip suitable for separating and analyzing urine cells and other visible components and an auxiliary reagent. The chip comprises the auxiliary reagent, which is a chemical mixed solution with sucrose concentration ranging from 5 to 50 percent and a buffer pH value of 7.4. The chip comprises a chip baseplate, a separation chip structure for storing urine, urine cells and other visible components in the chip, and a visible component separation buffer solution chip; and the outer part of the chip is linked with urine visible component collectors. 1. The chip for measuring urine cells and other visible components is characterized in that a chip auxiliary reagent skill storage chip is arranged outside a urine storage chip, and the framework of the chip is closely combined with the bottom cover and the upper cover of the chip. 2. The chip for separating urine cells and other visible components, as described in Claim 1 is characterized in that the urine storage chip and the separation solution chip both adopt a circular structure, four notches are formed in the framework of each chip, closely packed rubber isolating layers are arranged at the peripheries of the frameworks of the urine storage chip and the visible component separation solution chip, each rubber isolating layer is also provided with four notches, and after the rubber isolating layers are turned to superpose the notches, urine cells and other visible components can pass through the notches.

Description

Technical field [0001] The invention relates to a chip and related supporting reagents for measuring the separation of urine cells and formed components. More specifically, the present invention designs a separation chip for formed components such as urine cells, urine casts, and urine salt crystals, and is equipped with a separation reagent that can separate the cells, casts, etc. in urine. Organic formed elements are separated from salt crystals, etc., and are used in urine analysis equipment to classify and measure urine cells and casts. Background technique [0002] Red blood cells, white blood cells, epithelial cells, casts, etc. and urine salt crystals in urine all have a certain shape, and they are all formed elements in urine. The classification and quantity determination of red blood cells, white blood cells, large round epithelial cells, squamous epithelial cells, hyaline casts, cell casts, and salt crystals in urine have important clinical value for the diagnosis and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38B01D21/26
Inventor 孙续国
Owner 孙续国
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