Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for com-culturing and improving hydrogen output by utilizing bacteria and chlamydomonas reinhardtii

A rhinestone and co-cultivation technology, applied in the field of biological hydrogen production, can solve the problems of easy pollution and easy pollution of other bacteria, and achieve the effect of increasing hydrogen production

Inactive Publication Date: 2012-07-11
BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chlamydomonas reinhardtii is easy to contaminate other bacteria and other microorganisms during large-scale culture, and it is also easy to contaminate some bacteria during subculture in the laboratory, and some bacteria can coexist with Chlamydomonas reinhardtii for a long time without significantly affecting the growth of Chlamydomonas reinhardtii

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for com-culturing and improving hydrogen output by utilizing bacteria and chlamydomonas reinhardtii
  • Method for com-culturing and improving hydrogen output by utilizing bacteria and chlamydomonas reinhardtii
  • Method for com-culturing and improving hydrogen output by utilizing bacteria and chlamydomonas reinhardtii

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0041] (a), preparation of bacterial genome: take 2 mL of logarithmic growth phase bacterial solution, put it into a sterile centrifuge tube, and centrifuge at 12,000 r / min for 5 min. Discard the supernatant, add 300 μL of 10 mg / mL lysozyme to the centrifuge tube containing the bacterial pellet, and incubate for 2 hours. Then, 15 μL of 10 mg / mL RNAase, 30 μL of 20 mg / mL proteinase K, and 100 μL of 10% SDS were sequentially added, mixed well, and incubated at 65° C. for 45 minutes until clear. Then 125 μL of 5M NaCl and 100 μL of 10% CTAB were added and incubated at 65° C. for 30 minutes to fully lyse cells and remove polysaccharides. Then, according to the routine steps of DNA extraction, the DNA was extracted with phenol, the DNA was precipitated with absolute ethanol, and finally the DNA was dissolved in pure water for use.

[0042] (b), primer design: according to the literature, the general primers for bacterial 16S rDNA gene amplification were selected: 27F (5′-AGAGTTTGA...

Embodiment 1

[0060] Example 1 Cultivation of Chlamydomonas reinhardtii

[0061] Chlamydomonas reinhardtii is a representative species of photosynthetic hydrogen production by microalgae with fast growth rate, low culture cost and high hydrogenase activity. In order to make the experiment easy to operate, the present invention preferably prefers the cell wall-deficient Chlamydomonas reinhardtii sp. cc849.

[0062] ①Normal culture conditions of Chlamydomonas reinhardtii: According to "The Chlamydomonas Sourcebook: a comprehensive guide to biology and laboratory use. New York: Academic Press. 1989" edited by Harris, the optimal conditions are 25±1℃, and the illumination intensity of fluorescent light (100~200μmol photons) m -2 s -1 ); liquid culture is in 50~100ml Tris-Acetate-Phosphate (TAP) medium, initial pH 7.2, horizontal shaker speed 100~130rpm, 1% inoculation subculture every 5~6 days; solid TAP plate medium contains 1.5% agar powder, preservation and purification of algal species i...

Embodiment 2

[0065] Example 2 Isolation and identification of Chlamydomonas commensal bacteria

[0066] ①Isolation, purification and culture of Chlamydomonas commensal bacteria:

[0067] Take 1 mL of Chlamydomonas culture solution infected with bacteria at different growth stages and dilute it with sterile double distilled water for 10 -1 , 10 -2 , 10 -3 Three concentration gradients were applied to TAP medium, TAP+Escherichia coli medium LB (TAP medium in turn) [7] and E. coli LB medium [8] Mix in a 1:1 volume ratio) and YEM medium [9] On the solid plate of 25 ℃, 28-30 ℃ and 37 ℃ respectively, after the colony grows out, pick a single colony and subculture it in the corresponding liquid medium.

[0068] Using TAP+LB, YEM and TAP three medium plates, the bacteria commensal with Chlamydomonas reinhardtii cc849 were preliminarily isolated. As a result, two single colonies with different characteristics were isolated on the TAP+LB mixed medium, which were named L2 respectively. and L3; ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a biological hydrogen production technology, in particular to a method for com-culturing and improving hydrogen output by utilizing bacteria and chlamyodomonas reinhardtii. Through mixing aerobic bacteria represented by bradyrhizobium japonicum with the chlamydomonas reinhardtii according to a certain proportion for culture, the hydrogen output can be remarkably improved. The invention provides a new method for further improving the hydrogen output in a chlamydomonas reinhardtii twp-step-method hydrogen production technology.

Description

technical field [0001] The invention relates to a biological hydrogen production technology, in particular to a method for co-cultivating bacteria and Chlamydomonas reinhardtii to increase the hydrogen production. Background technique [0002] Energy crisis and environmental pollution are both prominent problems facing the world today. Hydrogen is a kind of clean, high combustion value and various forms of renewable bio-energy. [1] . Photosynthetic biological hydrogen production technology can directly convert solar energy into hydrogen energy, especially the substrate of microalgae photosynthetic hydrogen production is water, which is rich in source, easy to cultivate, and does not occupy arable land. Research hotspots in the field of biological hydrogen production [2] . Chlamydomonas reinhardtii was selected as the model species for the study of hydrogen production from microalgae due to its high hydrogenase activity, easy culture, fast growth, clear genetic background ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P3/00C12R1/01C12R1/38C12R1/89
Inventor 吴双秀
Owner BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com