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Dielectric cytometric apparatus and dielectric-cytometric cell sorting method

A cytometer and cell technology, applied in the field of cell sorting of dielectric cytometer, can solve different problems

Inactive Publication Date: 2012-07-04
SONY CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The big problem is that, strictly speaking, the original state of the cells used as controls is different from the state of the fluorescently labeled cells

Method used

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  • Dielectric cytometric apparatus and dielectric-cytometric cell sorting method
  • Dielectric cytometric apparatus and dielectric-cytometric cell sorting method
  • Dielectric cytometric apparatus and dielectric-cytometric cell sorting method

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Embodiment approach

[0179] The implementation of the present invention is by no means limited to the above-described embodiments. That is, various other embodiments may be implemented.

[0180] For example, in the above-described embodiments, a method based on a multipoint frequency is adopted as a method of measuring complex resistance. However, it is not necessary to adopt a multipoint frequency-based method as a method for measuring complex resistance.

[0181] In the above-mentioned embodiments, the frequency superposition method is adopted as a common multi-point frequency measurement. However, there are three other multipoint frequency measurement methods described below. Much like the frequency superposition method, in each of the three other multipoint frequency measurement methods, the distribution of individual cells through the narrow tract NC can be determined at a time in real time.

[0182] (1) Frequency scanning method

[0183] The frequency sweep method is a method of measurin...

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Abstract

Disclosed herein is a dielectric cytometric apparatus capable of analyzing cells and sorting the cells without adopting an optical analysis method and provides a dielectric cytometric cell sorting method for the apparatus. A stenosis channel allowing a single cell to flow is created on a cell injection section of a micro flow channel device used as a portion of the dielectric cytometric apparatus. A pair of measurement electrodes is created on the stenosis channel and an analyzer connected to the measurement electrodes measures the complex dielectric constant of for every cell passing through the stenosis channel. A electric-field application section provided on the downstream side of the stenosis channel applies an electric field for changing the flow of the cells inside a flow channel on the basis of information on the measured complex dielectric constants so that the cells can be sorted by making use of branch channels.

Description

technical field [0001] The invention relates to a dielectric cytometer device for analyzing and sorting cells and a cell sorting method of the dielectric cytometer. Background technique [0002] In the field of life science and medical research or in the field of medical care such as clinical practice, an analytical method known as flow cytometry is employed. In this flow cytometry, a fluid composed of cells free from each other is used as a sample. Under dilute conditions in which the average distance between cells is sufficiently larger than the cell size, the fluid serving as a sample is driven to flow through the inside of the flow channel tube. The signal detection part installed in the flow channel tube performs specific analysis / measurement on each cell flowing through the signal detection part. Cells whose measured signals are close to each other are considered to be of the same type. Therefore, the signals measured with respect to the number of cells contained in...

Claims

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Application Information

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IPC IPC(8): G01N27/02B07C5/344
CPCB03C5/005B01L2300/0645B03C5/028G01N15/12B01L2200/0652B01L2300/0864B01L3/502761B01L2400/0424G01N2015/1081B01L2300/0816G01N2015/1028
Inventor 大森真二佐藤一雅胜本洋一梅津友行林义人
Owner SONY CORP
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