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Screening method of medicines for inducing formation of myocardial cells by using model organism zebrafish

A cardiomyocyte and zebrafish technology, applied in the field of medicine, to achieve the effect of improving efficiency, high success rate, and high experimental accuracy

Inactive Publication Date: 2012-06-27
FUDAN UNIV
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Problems solved by technology

At present, zebrafish is mainly used for genetic development, and there is no related report on using it as a research object to screen small molecular compounds that can promote the formation of cardiomyocytes

Method used

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  • Screening method of medicines for inducing formation of myocardial cells by using model organism zebrafish
  • Screening method of medicines for inducing formation of myocardial cells by using model organism zebrafish
  • Screening method of medicines for inducing formation of myocardial cells by using model organism zebrafish

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Embodiment Construction

[0013] The present invention can be better understood from the following examples. However, those skilled in the art will easily understand that the source of the specific compound described in the implementation, the concentration, the selection of zebrafish and the results thereof are only suitable for illustrating the present invention, and should not and will not limit the detailed description in the claims. of the invention.

[0014] 1. Select an appropriate compound library or synthesize a new compound

[0015] The source of the compounds in this example is obtained from the high-throughput drug screening library of Vanderbilt University (Vanderbilt University) in the United States. 4000 compounds with different structures were obtained, and each small molecule compound was prepared into a 1mM stock solution with DMSO .

[0016] 2. Select a transgenic zebrafish strain relevant to the observation purpose

[0017] The purpose of this example is to observe small molecula...

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Abstract

The invention, belonging to the technical field of medicament, particularly relates to a screening method of medicines for inducing the formation of myocardial cells by using a model organism zebrafish, comprising the following steps: first, letting 25-30 pairs of Tg (cmlc2: EGFP) transgenic zebrafish homozygotes mate to obtain embryons, then transferring the embryons into a culture plate, adding a proper amount of zebrafish embryons at the stage of 50% epiboly in every pore, using an embryon culture solution containing 1-30 mu M small molecular compounds in an incubator for culture; then carefully checking the embryon heart size, heart shape and the number of myocardial cells; in addition, checking the whole shape (such as dorsal-ventral body axis, anterior-posterior body axis, etc.) and whether the formation of other organs of the embryons is influenced; and finally preliminarily judging whether the small molecular compounds promote the formation of the myocardial cells without influencing the growth of the other organs according to the experiment results. The new medicines which are screened out by the method has strong specificity and low toxic and side effect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to a high-content screening method for candidate drugs, in particular to a method for screening drug precursors (small molecular compounds) that promote the formation of cardiomyocytes using heart transgenic zebrafish as a model. Background technique [0002] Cardiovascular disease is the most common disease that endangers human health, and its morbidity and mortality have surpassed malignant tumors and ranked first. Despite the continuous progress of modern medicine, the treatment of ischemic heart disease such as myocardial infarction and heart failure still faces great challenges. The development of therapeutic methods that can stimulate the formation and regeneration of cardiomyocytes in the myocardial infarction area to increase the number of cardiomyocytes is clinically important. It has great medical prospects and economic benefits. The discovery of new biologica...

Claims

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Application Information

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IPC IPC(8): G01N33/15C12Q1/02
Inventor 钟涛
Owner FUDAN UNIV
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