Corn inbred line assistant seed selection method by using IDP molecular marker

Abstract

The invention relates to a corn inbred line assistant seed selection method by using an IDP molecular marker, which comprises the following steps: 1) screening 20 pairs of IDP primers possessing polymorphism to the corn inbred line seed selection base material S0 generation group; 3) using 10 pairs of IDP primers in the 20 pairs of IDP primers for screening S1 generation group to obtain an single plant of random n pairs of IDP primers lotus homozygosis in the above 10 pairs of IDP primers and obtain the inbred cluster; 3) using the IDP primers possessing polymorphism to the corn inbred line seed selection base material S0 generation group screened in the step 1) for removing the 20-n pairs of IDP primers outside the IDP lotus homozygosis used in the step 2), screening a S2 generation group to obtain the S2 generation group of the 20-n pairs of IDP primers lotus homozygosis which is the corn inbred line. According to the invention, the corn inbred line seed selection system with high efficiency and rapidity is established, and the inbred line seed selection time can be substantially shortened.

Description

technical field [0001] The invention belongs to the technical field of crop breeding, and relates to a method for breeding corn inbred lines, in particular to a method for assisting in breeding corn inbred lines by means of molecular markers. Background technique [0002] corn( Zea mays ) is an important raw material crop for modern food, feed, medicine and chemical industry. In recent years, corn production has developed rapidly and has become the third largest crop in the world. The sown area and total output are second only to rice and wheat. With the increase of population, the continuous development of animal husbandry and agricultural product processing industry, the demand for corn will continue to grow. my country is the second largest producer of corn in the world, and corn plays a very important role in my country's agricultural production and national economy. [0003] The existing methods for breeding maize inbred lines usually isolate selfed progeny that are ...

AI Technical Summary

Problems solved by technology

There are two main problems in the breeding of conventional maize inbred lines: first, the selection of maize inbred lines is mainly based on the work experience of the breeder and the observation of the phenotypic traits of the breeding materials. It is beneficial to control traits, but it is not good for quantitative traits controlled by polygenes, and most of the traits are controlled by polygenes, there are interactions between genes and they are highly susceptible to environmental influences

Second, the traditional method requires at least 6 to 8 selfing generations for the selection of basic materials, so the breeding cycle is relatively long and requires more human and financial resources.

[0006] The existing technology shows that there is no technical literature report on the selection of homozygous selection for maize inbred lines using IDP marker technology to segregate the offspring loci.

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