Transgenic helicoverpa armigera-resistant and phenol-free cotton breeding method

Abstract

The invention relates to a transgenic helicoverpa armigera-resistant and phenol-free cotton breeding method, which belongs to the cotton breeding field, and is characterized in that: eglandular high-yield low-phenol cotton specimen Hunan cotton No.18 with the gene model as Gl2eGl2e as a female parent to be hybrid with a male parent which is high-energetic fiber helicoverpa armigera-resistant specimen Jiangsu 76149. Due to the adoption of the method, the high energetic fibers can be quickly polymerized, high yield can be obtained, disease resistance can be realized, transgenic Bt insect resistance can be realized, a dominant phenol-free property can be realized, the selection efficiency can be remarkably improved, and the breeding period can be shortened. The new bred cotton specimen not only has stable resistance to the helicoverpa armigera and mice so as to reduce the application of helicoverpa armigera control medicine for 2 to 3 times, but also can reduce the production cost, and the cotton seed is safe and reliable to store and transport; and since no cotton phenol and similar substance are contained, the seeds of the cotton can be used as raw materials for processing human edible oil, food and healthcare products, so economic income of the cotton farmers can be increased for 1750 Yuan per Mu, and a promising popularization and application prospect in the cotton production region in China can be realized.

Description

technical field [0001] The invention relates to a method for cultivating transgenic cotton bollworm-resistant and phenol-free seed kernels, which belongs to the application field of cotton genetic improvement and biotechnology. Background technique: [0002] Cotton is an important fiber crop and economic crop. Among the harvested seed cotton, lint accounts for 40%, seed kernels account for 35%, seed shells account for 20%, and linters account for 5%. In addition to 30%-35% vegetable oil, the seed kernel also contains 40%-43% high-quality protein. The protein content of cotton kernel powder after seed oil extraction is as high as 60%, and is rich in 8 kinds of amino acids necessary for the human body. Its protein content and nutritional composition exceed those of rice, wheat, corn and peanuts. It is an important source of vegetable oil, and it is the vegetable protein resource second only to soybean in the world. However, at present, the cotton varieties cultivated on a l...

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of low phenol varieties being easily polluted by pollen of phenol varieties and lack of resistance to cotton field pests and mice in the practice of cotton production, by means of genetic improvement, using sexual hybridization combined with auxiliary selection of marker traits , Aggregate excellent traits such as insect resistance, low phenol, high yield, high-strength fiber, and disease resistance, cultivate new varieties of transgenic cotton bollworm resistance and dominant low phenol, develop and utilize the oil and edible functions of low phenol cotton seeds, and improve cotton planting Economic benefits, providing variety support for the successful transformation of cotton into grain, cotton and oil crops

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