Pure draft beer film cleaning and regenerating enzyme preparation and cleaning method using same
A technology for regenerating enzymes and enzyme preparations, applied in the field of fermentation engineering, can solve problems such as membrane fouling, ultrafiltration membrane clogging, and reduced membrane processing capacity, and achieve the effects of saving use costs, prolonging service life, and improving regeneration and recovery capabilities
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Embodiment 1
[0048] Preparation of Cleaning and Regenerating Enzyme and Membrane Cleaning in the Production of a Pure Draft Beer
[0049] Composition of raw materials: cleaning regeneration enzyme I, cleaning regeneration enzyme II.
[0050] Wash and regenerate enzyme I:
[0051] Pentosanase 375g, β-glucanase 100g, mannanase 25g.
[0052] Preparation method: mechanically mix pentosanase, β-glucanase, and mannanase according to the above proportions in a sterile environment.
[0053] Wash Regenase II:
[0054] Proline endoprotease 250g, neutral protease 250g.
[0055] Preparation method: mix the proline endoprotease and neutral protease according to the above ratio in a sterile environment.
[0056] Membrane cleaning:
[0057] A set of microporous filter membranes (0.45μm, 750mm, 36 pieces) filters pure draft beer according to production requirements, and then regenerates according to conventional acid-base cleaning methods: prepare 55°C hot water in the lye tank, pour it into the memb...
Embodiment 2
[0068] Preparation of cleaning regeneration enzyme and membrane cleaning in another pure draft beer production
[0069] Composition of raw materials: cleaning regeneration enzyme I, cleaning regeneration enzyme II.
[0070] Wash and regenerate enzyme I:
[0071] Pentosanase 375g, β-glucanase 200g, mannanase 25g.
[0072] Preparation method: mechanically mix pentosanase, β-glucanase, and mannanase according to the above proportions in a sterile environment.
[0073] Wash Regenase II:
[0074] Proline endoprotease 300g, neutral protease 200g.
[0075] Preparation method: mix the proline endoprotease and neutral protease according to the above ratio in a sterile environment.
[0076] Production test:
[0077] A set of microporous filter membranes (0.45μm, 750mm, 36 pieces) filters pure draft beer according to production requirements, and then regenerates according to conventional acid-base cleaning methods: prepare 55°C hot water in the lye tank, pour it into the membrane fi...
Embodiment 3
[0088] Preparation of cleaning regeneration enzyme and membrane cleaning in another pure draft beer production
[0089] Composition of raw materials: cleaning regeneration enzyme I, cleaning regeneration enzyme II.
[0090] Wash and regenerate enzyme I:
[0091] Pentosanase 315g, β-glucanase 315g, mannanase 70g.
[0092] Preparation method: mechanically mix pentosanase, β-glucanase, and mannanase according to the above proportions in a sterile environment.
[0093] Wash Regenase II:
[0094] Proline endoprotease 375g, neutral protease 125g.
[0095]Preparation method: mix the proline endoprotease and neutral protease according to the above ratio in a sterile environment.
[0096] Production test:
[0097] A set of microporous filter membranes (0.45μm, 750mm, 36 pieces) filters pure draft beer according to production requirements, and then regenerates according to conventional acid-base cleaning methods: prepare 55°C hot water in the lye tank, pour it into the membrane fil...
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