Preparation method of large-scale fungus test tube strain medium

A culture medium and test-tube seeding technology, applied in the field of microorganisms, can solve the problems of low nutrient content in the medium, slow germination of strains, and low production efficiency, and achieve the effects of fast mycelium growth, rapid germination, and improved efficiency

Inactive Publication Date: 2012-05-16
陈亮
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AI Technical Summary

Problems solved by technology

, to overcome the defects of low nutrient content in the medium, slow germination of strains, easy aging, high pollution rate, repeated expansion and transfer, high production cost and low production efficiency in the prior art

Method used

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Examples

Experimental program
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Embodiment Construction

[0024] (1) Take 100 catties of foxtail grass seeds, remove impurities and soak in water for 55 hours, then take out the grass seeds, dry the epidermis for later use;

[0025] (2) Preparation of nutrient solution: get 40% of soybean sprouts, 20% of wheat skin, and 40% of oyster mushrooms in fresh mushrooms and put them into water to boil (1: 1.5 by weight ratio of the three substances to water), keep warm for 40 minutes, and then Take out the liquid by filtration, and then heat and concentrate the liquid until the glass rod is inserted into the concentrated solution, and then the glass rod is lifted, until the rod hangs;

[0026] (3) adding potassium dihydrogen phosphate to the nutrient solution obtained in step (2), adjusting the pH to 7;

[0027] (4) Spray the grass seeds obtained in step (1) with the nutrient solution obtained in step (3) (the weight ratio of grass seeds and nutrient solution is 1: 0.3), and fully stir evenly, so that the surface of the grass seeds is comple...

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Abstract

The invention discloses a preparation method of a large-scale fungus test tube strain medium, which comprises the following steps: (1) collecting alopecuras aequalis sobol, removing impurities, soaking in water for 40 hours, taking grass seeds out, and drying in the air; (2) boiling bean sprout, wheat skin, and mushroom in water, performing heat preservation for 25 minutes, filtering to taking a solution out, heating and concentrating the solution; (3) adding potassium dihydrogen phosphate in the nutrient solution obtained in step (2), adjusting the PH to 6.5-7.2; (4) spraying the nutrient solution obtained in step (3) onto the grass seeds obtained in step (1), fully and uniformly stirring; (5) adding the medium obtained in step (4) into a test tube, plugging a cotton plug, binding the test tube up, putting the test tube in an autoclave, maintaining a gas pressure of 1.5 atm, performing sterilization for 60 minutes, naturally cooling to 70 degrees, opening the autoclave cover, taking out the test tube, when the temperature of the test tube decreases to about 25 DEG C, inoculating a basic fungal strain under an aseptic condition; (6) culturing the test tube strain obtained in step (5) at about 25 DEG C for 10-13 days so as to be ready for production.

Description

Technical field: [0001] The present invention relates to microorganisms, in particular to culture medium for macrofungi in microorganisms. Background technique: [0002] At present, the test-tube culture medium of macrofungi is mainly divided into solid medium and semi-solid medium. These two kinds of medium have their own advantages, but both of them have defects that are difficult to overcome. [0003] The nutrient content of the solid medium is low, the production is labor-intensive and time-consuming, the germination of the transferred original seed is slow, and the germination potential is weak. The medium of grains, wheat grains and rice is easy to be polluted and aged, and it is easy to cause pests and rodents after being transferred to the culture bag. . [0004] The disadvantage of semi-solid medium is that it is easy to age, which reduces the viability of mycelia and the production advantage declines. At room temperature, it often needs to be transferred multiple ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00
Inventor 陈亮
Owner 陈亮
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