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Macrofungus cuvette seed culture medium and method for producing the same

A culture medium and test-tube planting technology, applied in the field of microorganisms, can solve the problems of low nutrient content in the culture medium, low production efficiency, high production cost, etc., and achieve the effects of high production efficiency, good quality, and improved efficiency

Inactive Publication Date: 2008-05-07
张嘉闻
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a kind of culture medium for large-scale fungal test tubes, which overcomes the low nutrient content of the culture medium in the prior art, slow bacterial germination, easy aging, high pollution rate, and repeated expansion and rotation are required to produce Defects of high cost and low production efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Take 100 catties of foxtail grass seeds, remove impurities and soak in water for 55 hours, then take out the grass seeds, dry the epidermis for later use;

[0033] (2) Preparation of nutrient solution: get 30% of soybean sprouts, 15% of wheat skin, and 55% of oyster mushrooms in fresh mushrooms and put them into water to boil (1: 1.5 by weight ratio of the three substances to water), keep warm for 40 minutes, and then Take out the liquid by filtration, and then heat and concentrate the liquid until the glass rod is inserted into the concentrated solution, and then the glass rod is lifted, until the rod hangs;

[0034] (3) adding potassium dihydrogen phosphate to the nutrient solution obtained in step (2), adjusting the pH to 7;

[0035] (4) Spray the grass seeds obtained in step (1) with the nutrient solution obtained in step (3) (the weight ratio of grass seeds and nutrient solution is 1: 0.3), and fully stir evenly, so that the surface of the grass seeds is comple...

Embodiment 2

[0039] (1) Take 100 catties of foxtail grass seeds, remove impurities and soak in water for 50 hours, then take out the grass seeds, dry the epidermis for later use;

[0040] (2) Preparation of nutrient solution: get 20% of soybean sprouts, 10% of wheat skin, and 35% of oyster mushrooms of fresh mushroom species and put them into water to boil (1: 1 by weight ratio of the three substances to water), keep warm for 30 minutes, and then Take out the liquid by filtration, and then heat and concentrate the liquid until the glass rod is inserted into the concentrated solution, and then the glass rod is lifted, until the rod hangs;

[0041] (3) adding potassium dihydrogen phosphate to the nutrient solution obtained in step (2), adjusting the pH to 6.5;

[0042] (4) Spray the grass seeds obtained in step (1) with the nutrient solution obtained in step (3) (the weight ratio of grass seeds and nutrient solution is 1: 0.2), and fully stir evenly, so that the surface of the grass seeds is...

Embodiment 3

[0045] (1) Take 100 catties of Auranthus grass seeds, remove impurities, soak them in water for 60 hours, then take out the seeds, dry the epidermis for later use;

[0046] (2) Preparation of nutrient solution: get 40% of soybean sprouts, 30% of wheat skin, and 70% of oyster mushrooms in fresh mushrooms and put them into water to boil (1: 2 by weight ratio of the three substances to water), keep warm for 50 minutes, and then Take out the liquid by filtration, and then heat and concentrate the liquid until the glass rod is inserted into the concentrated solution, and then the glass rod is lifted, until the rod hangs;

[0047] (3) adding potassium dihydrogen phosphate to the nutrient solution obtained in step (2), adjusting the pH to 7.2;

[0048] (4) Spray the grass seeds obtained in step (1) with the nutrient solution obtained in step (3) (the weight ratio of grass seeds and nutrient solution is 1: 0.5), and fully stir evenly, so that the surface of the grass seeds is complete...

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Abstract

The invention discloses a large culture medium of epiphyte test tube and a preparation method. The culture medium is made by nutrient solution moistening amur foxtail or grass-seeds of green bristlegrass and the weight rate of the grass-seeds and the nutrient solution is 1 to 0.2 to 0.5. The surfaces of the grass-seeds are best moistened in and adhered with the nutrient solution. The test tube made by the culture medium can accelerate the growing speed of hypha and the time of the hypha filling with the test tube is two days earlier than the time when being grown in PDA, ten days earlier than the time when being grown in wooden meal and seven days earlier than the time when being grown in bamboo grain or wooden grain. The amount of seed grown in one test tube is equal to the amount of seeds grown in the test tube with PDA culture medium and going through three grades regeneration due that the grass-seed grain has the advantages of small volume, hard shell, good potential for growing epiphyte, low pollution as well as large surface and good dispersibility. The invention can be connected with the mushroom-produce bag and can reduce the culturing cycle by seventy days. The invention has the advantages of high producing efficiency, low production cost, low labor intensity and high commercial efficiency.

Description

Technical field: [0001] The present invention relates to microorganisms, in particular to culture medium for macrofungi in microorganisms. Background technique: [0002] At present, the test-tube culture medium of macrofungi is mainly divided into solid medium and semi-solid medium. These two kinds of medium have their own advantages, but both of them have defects that are difficult to overcome. [0003] The solid medium mainly includes sawdust medium, wood grain, bamboo grain medium, grain, wheat grain, and rice medium. Among them, the defects of wood chips, wood grain, and bamboo grain medium are: low nutrient content, labor-intensive and time-consuming production , the germination of the transferred original species is slow, the germination potential is weak, the medium of grains, wheat grains, and rice is easy to be polluted and aged, and it is easy to cause pests and rodents after being transferred to the culture bag. [0004] The semi-solid medium is mainly obtained b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 张嘉闻
Owner 张嘉闻
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