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Preparation method of olive leaf extract

The technology of olive leaf extract and olive dried leaf is applied in the field of preparation of olive leaf extract, can solve the problems of high cost, complicated extraction steps, low content of oleuropein, prevents purification and regeneration, and is suitable for scale Production, no environmental pollution effect

Inactive Publication Date: 2013-08-14
BANNERBIO NUTRACEUTICALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The technical problem to be solved by the present invention is to provide a simple and low-cost preparation method of olive leaf extract, aiming at the defects of complex extraction steps, high cost and low oleuropein content in the prior art, and using this method to obtain Oleuropein content in olive leaf extract of

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  • Preparation method of olive leaf extract
  • Preparation method of olive leaf extract
  • Preparation method of olive leaf extract

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preparation example Construction

[0042] Before utilizing the preparation method of the olive leaf extract to produce the olive leaf extract rich in oleuropein, the olive leaves are usually selected and pretreated.

[0043] Generally, affected by the growth cycle, the content of oleuropein in olive leaves picked in April and November is the lowest. Therefore, according to the present invention, in order not to greatly affect the content of oleuropein in the final extract, the The olive leaves are not collected during these two months. In addition, at other times of the year, although the content of oleuropein in olive leaves will fluctuate due to the influence of the growth cycle, it does not have much impact on the minimum content of oleuropein in the obtained olive leaf extract. In order to obtain higher content of oleuropein in the olive leaf extract, the olive leaves can also be collected in February or May according to the suggestion in Chinese patent CN101003557.

[0044] The fresh olive leaves collecte...

Embodiment 1

[0107] 200kg of dried olive leaves, after testing, the moisture content is less than 6%, and the oleuropein content is greater than 5%. Crumble olive leaves into coarse powder, heat and reflux extract twice with 1400L alcoholic 75% ethanol aqueous solution, each time for 4 hours, concentrate the extract after recovering ethanol, add 2000L water to the concentrated solution, add diatomaceous earth as an auxiliary material at the same time, mix Evenly, let it stand for 12 hours, press filter, put the filtrate on a 400kg D101 macroporous resin column, flow rate 0.5BV / h, desorb with an ethanol aqueous solution with an alcohol content of 70%, and concentrate in vacuo after recovering ethanol from the desorption solution, concentrate to 10°Bé, spray dry, Obtain 10.3kg of olive leaf extract, the yield is 5.15%, and the content of oleuropein is 61%. In this embodiment, the HPLC collection of illustrative plates of detecting the oleuropein content of olive leaf extract is as follows ...

Embodiment 2

[0109] 200kg of dried olive leaves, after testing, the moisture content is less than 6%, and the oleuropein content is greater than 5%. Crush part of the olive leaves into coarse powder, do not crush a small amount, moisten it with an appropriate amount of 70% ethanol aqueous solution to make it fully expand, pack it into a cylinder, flatten it evenly, open the piston of the percolate outlet, and add alcohol from the top 70% ethanol aqueous solution until the percolation liquid flows out from the outlet, and the solvent immerses the surface for several centimeters. After soaking for 24 hours, start percolation with 2000L of 70% ethanol at a flow rate of 100L / h, combine the percolation solution, recover ethanol from the percolation solution, add 1600L of water to the concentrated solution, add diatomaceous earth as an auxiliary material, mix well, and let it stand for 8 hours. Pressure filtration, 400kg D101 macroporous resin column on the filtrate, flow rate 0.5BV / h, desorptio...

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Abstract

The invention discloses a preparation method of an olive leaf extract. The preparation method comprises the following steps of: putting dried olive leaves into a polar solvent for extracting; recovering the solvent from an extracting solution, adding water and auxiliary materials, mixing uniformly, standing, filtering and combining filtrates; adsorbing a filtrate with macroporous resin, desorbingwith an ethanol-water mixed solution, and collecting a desorption solution; and recovering the solvent from the desorption solution, and drying to obtain the olive leaf extract. The method for preparing the olive leaf extract by adding auxiliary materials and purifying with the macroporous resin has the advantages of simple process, low cost and high content of oleuropein in the obtained olive leaf extract, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to the technical field of extraction of natural substances, more specifically, to a preparation method of an olive leaf extract, in particular to a preparation method of an olive leaf extract rich in oleuropein. Background technique [0002] Olive (Olea europaea L.), an evergreen tree belonging to the family Oleaceae (Oleaceae) and the genus Olea, is a world-renowned woody oil tree species with a cultivation history of more than 4,000 years. Olives are mainly distributed between 45° north latitude and 37° south latitude. The central distribution area is the Mediterranean countries. Greece, Italy, Tunisia, and Spain are the concentrated production areas. Now they are introduced and cultivated in all countries in the world. The olive used in traditional Chinese medicine in my country is the fruit of the olive plant olive (Canarium album Raeusch), also known as wild olive, which is not the same plant as the olive (Oleaceae), and the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/63A61K8/97C07H17/04A61P39/06A61P31/04A61P31/12A61P33/02A61P33/00A61P33/12A61P11/00A61Q19/00A61Q19/08A61Q17/04A23L1/30A61K127/00
Inventor 左小峰陈文良陈利琼周云
Owner BANNERBIO NUTRACEUTICALS
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