Immunohistochemical staining detection kit for colorectal cancer and application thereof
An immunohistochemistry and detection kit technology, which can be applied in measurement devices, scientific instruments, instruments, etc., and can solve the problems that the existence of tumors cannot be denied and the application scope is limited.
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[0044] An immunohistochemical staining kit for detecting colorectal cancer, the immunohistochemical staining kit comprising:
[0045] a) Anti-human Interleukin-17F (IL-17F) antibody derived from rabbits;
[0046] b) biotin-labeled goat anti-rabbit IgG;
[0047] c) avidin-labeled HRP;
[0048] d) normal goat serum blocking solution;
[0049] e) 3% (volume ratio) H 2 o 2 ;
[0050] f) DAB chromogenic solution;
[0051] g) hematoxylin;
[0052] b-g are products of Wuhan Boster Biological Engineering Co., Ltd.
[0053] Key in the present invention is the antibody preparation of the anti-human Interleukin-17F (IL-17F) of rabbit origin, and its method comprises the following steps:
[0054] A) Construction of IL-17F expression vector. Reagent test kit -Inducible / Secreted Kit with pCoHygro was purchased from Invitrogen. The Fc fragment tag of human immunoglobulin IgG1 was cloned into the pMT / BiP / V5-HisA expression plasmid in the kit to obtain the Drosophila expression sys...
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