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Separation method of rhamnolipid by using ultrafiltration membrane

A rhamnolipid and separation method technology, which is applied in the separation of rhamnolipid and the field of ultrafiltration membrane, can solve the problems of high energy consumption, difficulty in industrial scale-up, and low recovery rate, so as to reduce energy consumption and consumption Effect

Active Publication Date: 2014-09-17
浙江圣达紫金生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Among the above steps, high-speed centrifugation and evaporation concentration in (1), (4) and (6) will consume a lot of energy, and will greatly increase the initial equipment investment for separation; (3) will consume a lot of organic solvent, and it is easy to emulsify during the extraction process, prolonging the separation time, and the recovery rate is low; (5) the chromatographic separation requires a large amount of organic solvent as the eluent, the purification efficiency is extremely low, and it is not easy for industrial scale-up

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Using polyvinylidene fluoride (PVDF) hollow fiber ultrafiltration membrane to separate and purify rhamnolipids, the specific steps are as follows:

[0035] (1) Add 4% diatomaceous earth to the sterilized 550ml fermented broth containing 4% rhamnolipid, and then use a plate and frame filter press to filter at 0.6MPa to obtain 500ml of 30g / L removes bacterial body fermented liquid;

[0036] (2) Use concentrated hydrochloric acid to adjust the pH of the fermented broth to 4.0, and then use a PVDF hollow membrane with a molecular weight cut-off of 10KDa to perform ultrafiltration at 0.1MPa. When the cut-off volume is about 100ml, the first step of ultrafiltration is completed and collected Retained rhamnolipid concentrate;

[0037] (3) Add 20ml of methanol in situ to the retained rhamnolipid concentrate to make the final volume concentration of methanol at 18.9%, then adjust the pH of the solution to 9.0 with 1M NaOH, repeat the ultrafiltration operation (0.1Mpa), and col...

Embodiment 2

[0041] A method for separating rhamnolipids from a flat ultrafiltration membrane, the improved method comprising the following steps:

[0042] (1) The rhamnolipid content of the bacteria-free fermentation broth obtained after plate and frame filter press treatment (the amount of diatomaceous earth is 10% of the fermentation broth, and the operating pressure is 1.0Mpa) is 34 g / L;

[0043] (2) Take 1000ml of bacteria-free fermentation broth, adjust the pH to 5.0 with concentrated hydrochloric acid, and then perform ultrafiltration at room temperature and 0.1MPa using a polyethersulfone (PES) ultrafiltration flat membrane with a molecular weight cut-off of 300KDa. When the volume is about 200ml, the first step of ultrafiltration ends;

[0044] (3) Add 90ml of industrial ethanol to 200ml of ultrafiltration retentate, so that the final volume concentration of ethanol is 45%, and then adjust the pH of the solution to 9.0 with 1M NaOH, using the same PES hollow membrane as the first ...

Embodiment 3

[0048] Rhamnolipids were separated by polysulfone ultrafiltration roll-type membrane, and the specific steps and results were as follows:

[0049] (1) Take 1000mL of fermentation broth (containing 3.2% rhamnolipid) filtered by plate and frame pressure, adjust the pH to 5.0 with concentrated hydrochloric acid, and then use polysulfone (PSF) with a molecular weight cut-off of 10KDa at room temperature and 0.1MPa to The hollow membrane is used for ultrafiltration. When the cut-off volume is about 200ml, the first step of ultrafiltration ends;

[0050] (3) Add 86ml of propanol to 200mL of retentate to make the final volume concentration of propanol at 30%, then adjust the pH of the solution to 9.0 with 1M NaOH, and use the same set of polysulfone ultrafiltration roll-type membrane device for ultrafiltration. filter, collect the filtrate, and when the remaining volume is 47ml, the second step of ultrafiltration ends;

[0051] (4) Apply concentrated hydrochloric acid to the ultrafilt...

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Abstract

The invention discloses a two-step separation method for rhamnolipids by using an ultrafiltration membrane; in the two-step ultrafiltration method, in the first step, the pH of the fermented liquid for removing bacteria is adjusted to 4.0-6.0 to make the rhamnose The retention rate of lipid is above 95%. In the second step, add 15-45% (v / v) ethanol, and adjust the pH of the solution to 8.0-10.0 at the same time, and the permeability of rhamnolipid is above 90%. . After two-step ultrafiltration, the recovery rate of rhamnolipids is more than 80%. Two-step ultrafiltration can replace the existing traditional separation process of rhamnolipids, greatly reduce the amount of organic solvents, reduce energy consumption, Thereby reducing the cost of separation.

Description

technical field [0001] The invention relates to a membrane separation method, in particular to a separation method in which ultrafiltration membranes are applied to rhamnolipids. Background technique [0002] Rhamnolipid is a biosurfactant, which is mainly metabolized by Pseudomonas aeruginosa in a certain environment. It has good surface activity and interfacial activity, and can be widely used in petrochemical, environmental, and pharmaceutical industries. , food and agriculture. As the country pays more and more attention to environmental protection and restoration, and due to the outstanding performance of rhamnolipid in environmental restoration, its demand is increasing day by day. However, the high cost of separation greatly limits its wide application. usage of. [0003] The traditional separation of rhamnolipids involves the following steps: [0004] (1) Centrifuge the sterilized bacteria at high speed to remove the bacteria; [0005] (2) Adjust the pH of the fe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/04C07H1/08
Inventor 孟琴张国亮
Owner 浙江圣达紫金生物科技有限公司
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