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Novel method for producing food grade beta-amylase with ultrahigh activity

A production method, amylase technology, applied in the direction of hydrolytic enzymes, etc., can solve the problems of large waste of raw materials, environmental pollution, poor stability, etc., and achieve the effect of high comprehensive utilization rate, high purity, and high stability

Inactive Publication Date: 2012-12-26
淮北市三和诺生物工程有限责任公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there are also β-amylases extracted from plant materials such as wheat, barley, and soybeans in other places. The activity is generally between 100,000 and 500,000 units, the purity is not high, and the stability of the enzyme is poor, which cannot reach the industrial production of starch sugar. Ultra-high maltose syrup and maltitol requirements, and waste of raw materials, serious environmental pollution

Method used

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Experimental program
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Embodiment Construction

[0018] The new production method of food grade super high activity beta-amylase of the present invention, it is prepared according to the following process steps:

[0019] Step 1: Mix bran and water in a ratio of 1:5 (W / V), the water quality requirements: hardness ≤ 5mmol / L, pH value 7, temperature 20°C, extraction time controlled at 5 hours.

[0020] The second step: the supernatant of the first step is centrifuged at a speed of 1500 rpm for solid-liquid separation to remove impurities and flour in the solution.

[0021] Step 3: Add the solution separated in the second step into 5‰ polyacrylamide by volume, control the temperature at 5-35°C, pH value 4-7, stir for 20 minutes, fully react to make the miscellaneous protein get effective flocculation precipitation.

[0022] Step 4: Add 3% diatomaceous earth to the supernatant obtained after the flocculation in the third step, mix evenly, and further remove suspended solids and soluble starch in the solution through a plate-and-...

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Abstract

The invention discloses a novel method for producing food grade beta-amylase with ultrahigh activity. With bran as raw material, the method comprises the steps of digestion, centrifugation, flocculation, coarse filtering, fine filtering, ultrafiltration, secondary flocculation, fine filtering, ultrafiltration, tubular micro filtering disinfection, aseptic filling and the like. According to the invention, the produced beta-amylase has the advantages of ultrahigh purity activity, high purity, good stability and suitability for glucose industry, in particular for production field of ultra-high content maltose syrup and sugar alcohol; as agricultural and sideline products of flour processing, the bran has wide sources and the extracted bran can be still used as feed after being dried so that high comprehensive utilization rate is high. Therefore, very obvious competitive advantages are obtained by extracting the beta-amylase from the bran and very broad market prospect is obtained.

Description

Technical field [0001] The present invention relates to enzyme preparations, more specifically to a new method for the production of food-grade beta-amylase. Background technique [0002] In my country, the extraction of β-amylase from plants began in the late 1980s and early 1990s. It originated in Li County and Jin City, Hunan Province. It was extracted from bran. The extraction was mostly obtained by adsorption methods, and the activity of the enzyme preparation was low. In recent years, there are also β-amylases extracted from plant materials such as wheat, barley, and soybeans in other places. The activity is generally between 100,000 and 500,000 units, the purity is not high, and the stability of the enzyme is poor, which cannot reach the industrial production of starch sugar. Ultra-high maltose syrup and maltitol requirements, and waste of raw materials, serious environmental pollution. Contents of the invention [0003] The purpose of the present invention is to p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/24
Inventor 王传华
Owner 淮北市三和诺生物工程有限责任公司
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