Method for identifying tomato yellow leaf curl virus resistance

Abstract

The invention relates to a method for identifying tomato yellow leaf curl virus resistance, which enables infestive clonal construction of TYLCV in Tianjin region by aiming at a typical tomato yellow leaf curl virus sequence in Tianjin region. The resistibility of different tomato materials on the virus can be identified through inoculating tomato yellow leaf curl virus by injecting in stalks. The method avoids culturing Bemisia tabaci (Gennadius), ensures simple operation, high efficiency, high repeatability and high controllability, provides a relatively consistent inoculation pressure, obtains accurate result and is a simple and effective inoculation identification method.

Description

technical field The invention belongs to the technical field of plant protection, and relates to the research on inoculation identification of tomato yellow leaf curl virus, and more specifically relates to a rapid, simple and efficient method for identifying resistance inoculation of tomato yellow leaf curl virus in Tianjin area. Background technique Tomato yellow leaf curl virus (TYLCV) mainly harms vegetables such as tomato, pepper, eggplant, cabbage and cucumber, and cash crops such as tobacco, especially on tomato. For the occurrence and harm of TYLCV virus, how to detect the existence and occurrence of the virus accurately, quickly and efficiently is an urgent need for safe and sustainable vegetable production. At present, the methods for identifying the resistance of tomato yellow leaf curl disease include Bemisia tabaci transmission, grafting inoculation and mechanical transmission. Among them, the grafting method uses young diseased leaves as scions and inoculates...

AI Technical Summary

Problems solved by technology

Aiming at the problem that traditional techniques cannot accurately identify TYLCV resistance, the inventors have carried out a large amount of research work and constructed an invasive clone (code-named TYLCV--TJ) for TYLCV in Tianjin area, which was inoculated by stem injection Tomato yellow leaf curl virus, identification of resistance to this virus in different tomato materials

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