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Method for extracting anthocyanin type components from wine grape pomace

A technology of grape pomace and anthocyanins, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of high toxicity and side effects, low extraction rate of products, and achieve easy organization and no pollution to the environment , The process steps are simple and easy to implement

Inactive Publication Date: 2012-03-28
天津市尖峰天然产物研究开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The principle of this method is simple, and the requirements for equipment are low. The disadvantage is that most organic solvents have large toxic and side effects and low product extraction rate.
[0008] At present, the actual production and application of anthocyanins is limited to a few fields of health care and cosmetics. There are still many accurate physiological functions of anthocyanins in plants that have yet to be further studied. As an active ingredient that exists widely in plants, further research Its physiological role and metabolic characteristics in the human body, and its application in medical care after its molecular modification or improvement of its properties will be a hot issue in the future research of anthocyanins

Method used

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  • Method for extracting anthocyanin type components from wine grape pomace

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Evenly spray 10% of the total weight of purified water in the wine grape pomace, and ferment naturally in the extraction tank at 37-39°C for 40 hours;

[0032] (2) With 45% (volume fraction) ethanol that is 12 times the weight of raw materials, heat reflux extraction twice, each time for 2 hours;

[0033] (3) The two extracts are combined, concentrated in a vacuum, and ethanol is recovered to obtain the grape skin extract liquid;

[0034] (4) Extract the medicinal solution and pass through the AB-8 macroporous resin chromatography column, rinse with deionized water, elute with 2.5 times the column volume of 60% (volume fraction) ethanol aqueous solution, and collect the eluate;

[0035] (5) The eluate was concentrated in vacuum to a sugar content of 42°;

[0036] (6) The concentrated paste was spray-dried to obtain a product with an anthocyanin content of 9.76%.

Embodiment 2

[0038] (1) Evenly spray 10% of the total weight of purified water in the wine grape pomace, and ferment naturally in an extraction tank at 39-42°C for 36 hours;

[0039] (2) With 55% (volume fraction) ethanol that is 10 times the weight of raw materials, heat reflux extraction twice, each time for 2 hours;

[0040] (3) The two extracts are combined, concentrated in a vacuum, and ethanol is recovered to obtain the grape skin extract liquid;

[0041] (4) The extracted medicinal solution is passed through a polyamide chromatography column, washed with deionized water, eluted with 70% (volume fraction) ethanol aqueous solution twice the column volume, and the eluate is collected;

[0042] (5) The eluate was concentrated in vacuum to a sugar content of 42°;

[0043] (6) The concentrated paste was spray-dried to obtain a product with anthocyanin content of 12.36%.

Embodiment 3

[0045] (1) Evenly spray 20% of the total weight of purified water in the wine grape pomace, and ferment naturally in the extraction tank at 39-42°C for 36 hours;

[0046] (2) With 65% (volume fraction) ethanol that is 12 times the weight of raw materials, heat reflux extraction twice, each time for 2 hours;

[0047] (3) The two extracts are combined, concentrated in a vacuum, and ethanol is recovered to obtain the grape skin extract liquid;

[0048] (4) Extract the medicinal solution through the X-5 resin chromatography column, rinse with deionized water, elute with 4 times the column volume of 50% (volume fraction) ethanol aqueous solution, and collect the eluent;

[0049] (5) The eluate was concentrated in vacuum to a sugar content of 42°;

[0050] (6) The concentrated paste was spray-dried to obtain a product with an anthocyanin content of 8.34%.

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Abstract

The invention discloses a method for extracting anthocyanin type components from wine grape pomace. The method comprises the following steps: uniformly spraying 10% of the total weight of purified water in the wine grape pomace, naturally fermenting at the temperature of 35-41 DEG C in an extraction tank for 36 hours, and then performing reflux extraction with water-containing ethanol twice with 2 hours per time; recovering the ethanol in extract solution, passing through an adsorption type macroporous resin chromatographic column, flushing with deionized water, using ethanol water solution for elution, and collecting eluate; and performing vacuum concentration till the sugar degree is 42 degree, and performing spray-drying for getting a product. By adopting the method, wine-making waste, namely the wine grape pomace in the grape planting industry and grape wine baking enterprises can be fully utilized, and the waste of waste resources in the agriculture and the manufacturing industry can be avoided. Only the water and the ethanol are used in the extraction process in the method, the method does not use any organic solvent, inorganic acid or SO2 and the other color fixatives, the preparation process does not pollute the product or an environment, and a safe and pollution-free grape skin anthocyanin type extract is provided.

Description

technical field [0001] The invention relates to a method for extracting anthocyanins from wine grape pomace, especially a method suitable for large-scale production, which is safe and non-polluting by fully utilizing agricultural and manufacturing wastes through macroporous resin column chromatography technology. Methods of grape anthocyanin constituents. Background technique [0002] Grape, Vitis (Vitis) deciduous vine, palmate leaves, 3-5 lobed, complex racemes, usually conical, berries are mostly round or oval, and the color varies with the variety. Humans began to cultivate this fruit tree a long time ago, accounting for almost a quarter of the world's fruit production; it has high nutritional value and can be made into grape juice, raisins and wine. [0003] Anthocyanins are a class of compounds formed by the combination of anthocyanins and sugars through glycosidic bonds. They are widely present in the cell fluids of flowers, fruits, stems, leaves and root organs of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07H17/02C07H1/08C09B61/00
Inventor 孙华庚
Owner 天津市尖峰天然产物研究开发有限公司
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