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Method for purifying natural serine protease inhibitors

A technology of serine protease and purification method, which is applied in the field of purification of natural serine protease inhibitors, can solve the problems of lack of purification methods of natural serine protease inhibitors, etc., and achieves the effects of no toxic and side effects, high yield and easy operation.

Inactive Publication Date: 2012-03-14
特菲(天津)生物医药科技有限公司 +1
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Problems solved by technology

But at present, there are basically no products developed using the active ingredients in silkworm chrysalis as drugs in the market, and there is a lack of purification methods for natural serine protease inhibitors

Method used

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Embodiment Construction

[0011] A method for purifying natural serine protease inhibitors. Including the following steps: (1) pretreatment of silkworm chrysalis, fresh silkworm chrysalis and 50mM phosphate buffer solution pre-cooled at 4°C, pH 7.4, were added to a juice extractor at a ratio of 1:1 to homogenate for 30 seconds, and placed on ice for 5 Minutes, continue homogenization, repeat three times to fully dissolve the protein in silkworm chrysalis; transfer the homogenate to a centrifuge cup, 4°C, 15,000 rpm, centrifuge for 20 minutes, take the supernatant, and pass the supernatant through nine layers of gauze Filter to remove grease, repeat the centrifugation and filtration steps three times; collect the filtered supernatant, centrifuge at 4°C at 15,000 rpm for 30 minutes, vacuum filter the supernatant with a 0.45 μm filter membrane, and store at 4°C. (2) Preliminary separation and purification of natural serine protease inhibitors, the supernatant was placed in a constant temperature water bat...

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Abstract

The invention belongs to a method for purifying natural serine protease inhibitors, which comprises the steps of silkworm pupa pretreatment, primary separation purification of the natural serine protease inhibitors, and the separation purification of the natural serine protease inhibitors. The method has the advantages that silkworm pupas are used as raw materials, the natural serine protease inhibitors with the medicinal value in the silkworm pupas are obtained through separation and purification, the method is simple and convenient to operate, the yield is high, the method is suitable for large-scale production, the purified natural serine protease inhibitors can be used as health-care medicine, and no toxic or side effect is caused.

Description

technical field [0001] The invention belongs to a method for purifying natural serine protease inhibitors. Background technique [0002] Silkworm chrysalis is the chrysalis left after the silkworm cocoon silkworm of silk moth family insect silkworm. It has a wide range of sources and is considered to be a new source of nutrition for humans. It is the only insect food in the "New Food Resource List for General Food Management" approved by the Ministry of Health. Silkworm chrysalis has extremely high nutritional value and is rich in protein, fatty acids and vitamins. The serine protease inhibitor (serpin, abbreviated as SP) contained therein can be used as a protease inhibitor to participate in coagulation reaction, fibrinolysis, angiogenesis, complement activation, immune regulation and inflammatory response, etc. Still a few perform non-inhibitory functions: acting as hormone transporters, molecular chaperones and tumor suppressors. They play a key regulatory role in vari...

Claims

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Application Information

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IPC IPC(8): C07K14/81C07K1/14
Inventor 叶曼陈昊张耀洲陈剑清舒特俊
Owner 特菲(天津)生物医药科技有限公司
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