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Transformation method of rice cells

A rice and cell technology, applied in the field of plant genetics and genetic engineering, can solve the problems of low transformation efficiency and unsuitable for industrial-scale production, and achieve the effects of reducing environmental pressure, high repeatability, and shortening time-consuming

Active Publication Date: 2013-12-04
SYNGENTA BIO TECH CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The pmi selection system has also been used in rice transformation research (Lucca et al.2001; He et al.2004; Ding et al.2006), but the reported transformation efficiencies are very low (only about 6%), which is not suitable for production on an industrial scale

Method used

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Embodiment Construction

[0065] The present invention will be further described below in combination with specific embodiments. It should be understood that the specific implementations described here are merely examples to help those skilled in the art better understand the present invention, and do not constitute any limitation to the scope of the present invention.

[0066] In the absence of other specific instructions, the experimental methods and operations in the following specific embodiments are all carried out using common routine operations in the art. Those skilled in the art can easily obtain teachings about such routine operations from the prior art, for example, with reference to the textbook Sambrook and David Russell, Molecular Cloning: A Laboratory Manual, 3rd ed., Vols 1, 2; Charles Neal Stewart , Alisher Touraev, Vitaly Citovsky and Tzvi Tzfira, Plant Transformation Technologies, etc.

[0067] "About" or "around" mentioned in this article, unless otherwise specified or clearly conf...

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Abstract

The invention provides a method of effectively introducing exogenous genes into rice cells by utilizing PMI (phosphomannose isomerase) selective marker, a transformation system, as well as a preparation method of genetically modified rice by utilizing the method.

Description

technical field [0001] The invention relates to the fields of plant genetics and genetic engineering. More specifically, the present invention relates to a method for preparing transgenic rice using Agrobacterium-mediated and PMI selectable markers. Background technique [0002] Rice (Oryza sativa) is the staple food for more than half of the world's population, especially in Asian countries. As the population expands and arable land decreases, rice production is becoming increasingly difficult to meet demand. By 2050, the world population will increase from the current 6.7 billion to 9.2 billion (United Nations, 2007), which requires an additional 50% increase in food supply by 2050. With the popularization of semi-dwarf varieties and hybrid rice, transgenic methods are expected to become another breakthrough point for increasing rice production. After the completion of the rice genome sequencing in 2002, the functional annotation of open reading frames and the applicati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82
Inventor 翟晨光段永波李浩章旺根
Owner SYNGENTA BIO TECH CHINA
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