Preparation method and use method of loach aeromonas sobria vaccine

Abstract

The invention discloses a preparation method of a loach aeromonas sobria vaccine, which is characterized by comprising the following step: preparing a water aqua type loach aeromonas sobria vaccine using radix astragali as an adjuvant by using loach pathogenic aeromonas sobria LD081008A-1 as immunogen and a radix astragali lixivium as the adjuvant, wherein the concentration of the pathogenic aeromonas sobria as the immunogen in the vaccine is 2.7*108 cells / mL, and the concentration of the radix astragali lixivium is 1 g / mL. The invention further discloses a use method of the loach aeromonas sobria vaccine. By using the loach aeromonas sobria vaccine prepared by the method, the capability of the resistance of loaches to aeromonas sobria infection can be obviously improved; the immune protecting capability of the loaches to the pathogenic aeromonas sobria is enhanced; the survival rate of cultivating loaches is improved; the yield of cultivating the loaches is increased; the export obstacle caused by drug residue is avoided; the economic benefit in the cultivation production of the loaches is improved; and the problem that diseases caused by the aeromonas sobria easily appearing in the current cultivation production process of the loaches is solved foundationally.

Description

technical field [0001] The invention relates to a preparation method of an animal disease-resistant vaccine, in particular to a preparation method of a loach Aeromonas temperis vaccine. The invention also relates to methods of using the vaccine. Background technique [0002] Aeromonas sobria widely exists in the water environment and is distributed all over the world. It is an opportunistic pathogen and can cause various diseases of fish and other aquaculture animals, causing serious economic losses to the aquaculture industry. Therefore, the efficient prevention of the pathogenic Aeromonas temperatus, in order to minimize its harm, will significantly improve the economic benefits of loach farming. In terms of the prevention and control of bacterial diseases in farmed fish, chemically synthesized drugs or antibiotics are mostly used to control the occurrence and prevalence of diseases in aquaculture production. However, in the long-term use of drugs, people have realized th...

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Problems solved by technology

In terms of the prevention and control of bacterial diseases in farmed fish, chemically synthesized drugs or antibiotics are mostly used to control the occurrence and prevalence of diseases in aquaculture production. However, in the long-term use of drugs, people have realized that there are many shortcomings in drug treatment: 1. The etiology is complicated, there is blindness in a large number of medications, and there are no targeted effective drugs for many diseases; the second is that some chemical drugs have a narrow effective range and weak preventive effect, and it is often difficult for sick fish to ingest effective drug doses due to anorexia; Residues in animals endanger human health, and long-term medication brings environmental pollution and leads to a large number of drug-resistant microorganisms; Individuals survived and their proportion in the population gradually increased, resulting in a decline in the disease resistance of the entire population, which seriously affected the development of the aquaculture industry.

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