Endophytic fungus for producing triptolide

Abstract

The invention provides an endophytic fungus for producing triptolide. The endophytic fungus is a Penicillium steckii strain NS-12 and is collected in China General Microbiological Culture Collection Center, and the collection number is CGMCC No. 4928. Fermentation culture conditions for producing the triptolide by using the endophytic fungus are that: a potato dextrose agar (PDA) liquid culture medium (the liquid loading capacity is 100ml / 500ml) is used, the shake culture is performed by a shaker at the rotating speed of 150r / min, the culture temperature is 28 DEG C, and the culture time is 72 to 144h. Through suspension culture, the strain can secrete a considerable amount of triptolide out of cells in a short time; the method is remarkably superior to an acquisition way of the traditional plant cultivation; and the strain has good industrialized application prospect.

Description

technical field [0001] The invention relates to an endophytic fungus producing triptolide. Background technique [0002] Since Vogl isolated the first strain of endophytic fungi from ryegrass seeds in 1898, plant endophytes have received extensive attention as a new microbial resource, and finding and discovering new active compounds from endophytes has become a Another hot spot of research at home and abroad. Studies have shown that endophytic fungi exist in all plant species, and endophytic fungi in medicinal plants can produce the same or similar medicinal active ingredients as host plants, especially many new active ingredients have been found, which have been proven to have Obvious anticancer, antibacterial, antioxidant and other activities. With the continuous deepening of research on medicinal plant resources, some endophytic fungi parasitic in medicinal plants have been found to not only significantly promote the production of host secondary metabolites, but also p...

AI Technical Summary

Problems solved by technology

However, the content of medicinal components of Tripterygium wilfordii is extremely low, coupled with the small reserves of wild resources, and artificial cultivation has the problems of slow growth and long harvest period, which is far from meeting market demand and has become a precious and scarce Chinese herbal medicine in my country.

Therefore, the extraction of the endophytic fungus of Tripterygium wilfordii has very important significance, but the research in this area has not been reported.

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