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Preparation method of total flavones of artemisia anomalas

A technology of total flavonoids and Artemisia annua is applied in the field of preparation of Artemisia annua total flavonoids, which can solve the problems of large amount of solvent, long extraction time, and no known total flavonoids of Artemisia annua, and achieves less solvent consumption and good reproducibility. , the effect of improving economic and social benefits

Inactive Publication Date: 2012-01-11
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the method for extracting Artemisia flavonoids has disadvantages such as long extraction time and large amount of solvent used, and there is no related patent on the preparation method of Artemisia flavonoids in China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Grind the dried Artemisia annua, take 2kg, put it into the extraction tank, and use ethanol as the carrier agent, the amount of the carrier agent is 25% of the amount of the extracted material, set the temperature of the extraction kettle to 42°C, and the temperature of the analysis kettle to 40°C. The pressure is 28MPa, CO 2 The flow rate is 30L / h. After 2 hours of extraction, the extract is analyzed. The pressure of the analysis tank is 6MPa. The extract is dispersed with water to obtain a sample solution of 0.8 mg / ml. The AB-8 macroporous resin is used for dynamic adsorption. After the adsorption is completed , first washed with 8L of water to remove water-soluble impurities, and then eluted with 65% ethanol, the eluent was concentrated under reduced pressure, and freeze-dried to obtain 56g of the total flavonoids product of Artemisia annua.

[0014]

Embodiment 2

[0016] Grind the dried Artemisia annua, take 2kg, put it into the extraction tank, and use ethanol as the carrier agent. The pressure is 30MPa, CO 2 The flow rate is 25L / h. After 2.5 hours of extraction, the extract is analyzed. The pressure of the analysis tank is 6.5MPa. The extract is dispersed with water to obtain a sample solution of 1.2 mg / ml. S-8 macroporous resin is used for dynamic adsorption. After completion, first wash with 10L of water to remove water-soluble impurities, then elute with 65% ethanol, concentrate the eluate under reduced pressure, and freeze-dry to obtain 52 g of the total flavonoids product of Artemisia annua.

[0017]

Embodiment 3

[0019] Grind the dried Artemisia annua, take 2kg, put it into the extraction tank, and use ethanol as the carrier agent, the amount of the carrier agent is 25% of the amount of the extracted material, set the temperature of the extraction kettle to 35°C, the temperature of the analysis kettle to 30°C, and the extraction kettle The pressure is 35MPa, CO 2 The flow rate is 38L / h. After 3 hours of extraction, the extract is analyzed. The pressure of the analysis tank is 6MPa. The extract is dispersed with water to obtain a sample solution of 1 mg / ml, which is dynamically adsorbed by ADS-7 macroporous resin. After the adsorption is completed, First wash with 8L of water to remove water-soluble impurities, then elute with 60% ethanol, concentrate the eluate under reduced pressure, and freeze-dry to obtain 63g of the total flavonoids product of Artemisia annua.

[0020]

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PUM

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Abstract

The invention discloses a preparation method of total flavones of artemisia anomalas, belonging to an extractivetechnique of total flavones, comprising the following steps: crushing dry artemisia anomalas, using the supper critical carbon dioxide fluid extraction technology and ethanol as carrying agent to obtain the extract, absorbing the extract with macroporous resin, carrying out elution with ethanol, carrying out concentration under reduced pressure, and carrying out freeze drying to obtain a product of total flavones of artemisia anomalas. The method has the advantages of simple and rapid operation without toxicity, and is a green production method.

Description

technical field [0001] The invention belongs to the field of separation and purification of natural products, and relates to a preparation process of effective substances in natural products, in particular to a preparation method of total flavonoids of Artemisia annua. Background technique [0002] Artemisia argyi Artemisia anomala S. Moore, using the whole herb with flowers as medicine. It has the effects of clearing away summer-heat and promoting dampness, promoting blood circulation and promoting blood stasis, stimulating menstrual flow and relieving pain. For heatstroke, headache, enteritis, dysentery, abdominal pain due to amenorrhea, rheumatism pain, bruises; external use for wound bleeding, mastitis. Artemisia annua contains flavonoids, coumarins, sesquiterpene lactones and organic acids. The total flavonoid content of Artemisia annua is about 6%. [0003] At present, the method for extracting Artemisia flavonoids has disadvantages such as long extraction time an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/282A61P1/00A61P7/04A61P15/00A61P15/14A61P25/04A61P29/00A61P43/00
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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