A kind of fir tissue culture method

Abstract

The invention provides a Chinese fir tissue culture method. The culture method adopts open tissue culture, and the bacteriostatic agent used is one or more of Junsha Baoguo, mancozeb and sodium hypochlorite. The method of this invention can reduce the pollution rate to an acceptable range without affecting the effect of tissue culture. The tissue culture of plants in a bacterial environment fundamentally simplifies the tissue culture process and reduces the cost of tissue culture.

Description

technical field [0001] The invention relates to a fir tissue culture method. Background technique [0002] Chinese fir( Cunninghamia lanceolata ) is the most important fast-growing and high-yielding tree species in southern my country. It has excellent characteristics such as fast-growing, high-yielding, good material properties, and wide application. It has an important position in my country's plantation forests. Since Chinese fir is a cross-pollinated tree species, it is propagated by seeds, and the individual differentiation is large, so it is difficult to maintain the traits of the Chinese fir parent body; and conventional asexual reproduction methods such as cutting, layering or grafting are slow in breeding and difficult to meet the growing market demand. The tissue culture method is a rapid vegetative propagation technique. Selecting excellent Chinese fir clone materials and adopting the tissue culture method can not only maintain the excellent traits of the mot...

AI Technical Summary

Problems solved by technology

However, the complicated technical links in the traditional tissue culture process are all formulated around pollution prevention. Pollution control has become the primary technology in tissue culture, which is the main reason for the high price of Chinese fir tissue culture seedlings

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