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A kind of artificial cultivation method of Brown Ganoderma lucidum fruiting body

A kind of Ganoderma lucidum fruiting body, artificial cultivation technology, applied in the artificial cultivation field of Brown Ganoderma lucidum (Gilb.) fruiting body, can solve the problems such as unsuccessful artificial cultivation method, limited wild resources and the like

Active Publication Date: 2011-12-21
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Brown Ganoderma lucidum is often used as a substitute for Ganoderma lucidum, but its wild resources are very limited, and there is no successful artificial cultivation method so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Weigh 75g of peeled and removed potatoes, 6g of sucrose, 6g of agar, and 3g of pine needles. Cut the potatoes into small strips and put them in a pot with pine needles. Add 405g of water and boil for 20 minutes until the potatoes are soft but not rotten. Filter with 6 layers of gauze, take the filtered juice in a pot, add water to make up to the original water volume, add agar to melt, add sucrose and stir evenly, adjust the pH to 6, put it in a test tube while it is hot and sterilize, cool to 45°C and pour it into a slope, Obtain slant PDA culture medium;

[0022] Weigh 75g of peeled and removed potatoes, 6g of sucrose, 6g of agar, 0.2g of potassium dihydrogen phosphate, 0.2g of magnesium sulfate and VB 1 0.002g, cut the potatoes into small strips and put them in the pot, add 306g of water, boil for 20 minutes until the potatoes are soft but not rotten, filter with 6 layers of gauze, take the filtered juice in the pot, add water to the original water level, add Melt t...

Embodiment 2

[0032] Weigh 850g of peeled and removed potatoes, 100g of sucrose, 100g of agar, and 50g of pine needles. Cut the potatoes into small strips and put them into the pot with pine needles. Add 3850g of water and boil for 30 minutes until the potatoes are soft but not rotten. Filter with 8 layers of gauze, take the filtered juice in the pot, make up to the original amount of water, add agar to melt, then add sucrose and stir evenly, adjust the pH to 8, put it in a test tube while it is hot and sterilize, cool to 50°C and pour it into a slope , to obtain slanted PDA medium.

[0033] Weigh 850g of peeled and removed potatoes, 100g of sucrose, 100g of agar, 15g of potassium dihydrogen phosphate, 15g of magnesium sulfate and VB 1 0.06g, cut the potatoes into small strips and put them in the pot, add 4905g of water, boil for 30 minutes until the potatoes are soft but not rotten, filter with 8 layers of gauze, take the filtered juice in the pot, make up to the original amount of water, ...

Embodiment 3

[0043] Weigh 800g of peeled and removed potatoes, 80g of sucrose, 80g of agar, and 40g of pine needles. Cut the potatoes into small strips and put them into the pot with pine needles. Add 4000g of water and boil for 25 minutes until the potatoes are soft and not rotten. Filter with 7 layers of gauze, take the filtered juice in the pot, make up to the original amount of water, add agar to melt, then add sucrose and stir evenly, adjust the pH to 7, put it in a test tube while it is hot and sterilize, cool to 48°C and pour it into a slope , to obtain slanted PDA medium.

[0044] Weigh 800g of peeled and removed potatoes, 80g of sucrose, 80g of agar, 10g of potassium dihydrogen phosphate, 10g of magnesium sulfate and VB 1 0.04g, cut the potatoes into small strips and put them in the pot, add 3920g of water, boil for 25 minutes until the potatoes are soft but not rotten, filter with 7 layers of gauze, take the filtered juice in the pot, make up to the original amount of water, Add...

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PUM

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Abstract

The invention relates to an artificial cultivation method for Ganoderma brownii (Murrill) Gilb. fruiting bodies, which is characterized in that tissue isolation or spore isolation is conducted to fresh Ganoderma brownii (Murrill) Gilb. fruiting bodies, the isolated tissues or spores are cultured on a potato dextrose agar (PDA) inclined-surface slant culture medium and a slant comprehensive PDA culture medium to obtain stock culture for production, the stock culture for production is inoculated and cultured in a pre-culture spawn culture medium to obtain pre-culture spawns, the pre-culture spawns are cultured in a production spawn culture medium to obtain production spawns, and finally the production spawns are inoculated in a culture medium for production and are cultured till mature fruiting bodies grow. The artificial cultivation method disclosed by the invention has the advantages that the biological efficiency of the Ganoderma brownii (Murrill) Gilb. fruiting bodies can reach 30 percent to 90 percent, i.e. 30g to 90g fresh Ganoderma brownii (Murrill) Gilb. fruiting bodies can be obtained per 100g culture medium, and the fresh Ganoderma brownii (Murrill) Gilb. fruiting bodies can be used as raw materials or materials for researches.

Description

technical field [0001] The invention relates to an artificial cultivation method of ganoderma brownii (Murrill) Gilb., in particular to an artificial cultivation method of Ganoderma brownii (Murrill) Gilb. Background technique [0002] Ganoderma lucidum resources in China are very rich, and there are more than 100 known species, and there are no less than 20 kinds of Ganoderma lucidum that can be used in folk or literature records, among which Ganoderma lucidum (Curtis) P.Karst., Ganoderma lucidum (Ganoderma sinenseJ. D.Zhao, L.W.Hsu & X.Q.Zhang) and Ganoderma tsugae Murrill as representatives. Because these Ganoderma lucidum contains rich pharmacological components such as Ganoderma lucidum polysaccharides, Ganoderma lucidum polypeptides, triterpenoids, amino acids, proteins, steroids, mannitol, coumarin, alkaloids, organic acids and trace elements Ge, P, Fe, Ca, Mn, Zn, etc., thus have the effects of inhibiting tumors, sedative and analgesic, immune regulation, anti-radia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 宋斌李泰辉邹方伦吴兴亮
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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