Method for simultaneous detection of zearalenone and fumonisins

Abstract

A method for simultaneously detecting zearalenone and fumonisins in the technical field of biological detection engineering, comprising the following steps: 1. Prepare conjugates ZEN-BSA and ZEN-OVA; FB1-KLH and FB1-OVA; 2. Prepare anti-ZEN monoclonal antibody and anti-FB1 monoclonal antibody respectively; 3. Prepare colloidal gold, respectively label anti-ZEN monoclonal antibody and anti-FB1 monoclonal antibody; spray the labeled monoclonal antibody onto the gold label pad ; 4. Spotting on the nitrocellulose membrane; 5. Assemble into a test strip; 6. Drop the methanol extraction supernatant of the sample to be tested into the sample groove of the test strip to obtain the points on the nitrocellulose membrane. The color development result of the sample band, the identification, and the result are obtained. The detection object of the method of the present invention is highly targeted and has high accuracy; the detection speed is fast and the required time is short, the loss of manpower and material resources caused by using two detection methods to detect two toxins is reduced, and it is convenient for promotion and application at the grassroots level.

Description

technical field [0001] The invention relates to a method in the technical field of biological detection engineering, in particular to a method using a nano colloidal gold immune layer [0002] A method for the simultaneous detection of zearalenone and fumonisins by analytical technology. Background technique [0003] Zearalenone (ZEN) is a secondary metabolite produced by some strains of Fusarium under certain humidity and temperature conditions. Zearalenone can be detected in grains such as wheat, barley, corn, rye, sorghum, and can also be detected in some animal tissues or products (such as milk, eggs, etc.). Zearalenone toxin can cause a variety of diseases in humans and animals, mainly carcinogenic and cancer-promoting toxicity. Reproductive system diseases have an important relationship. Zearalenone toxin has the characteristics of widespread distribution, long residual time, difficult to handle, and enhanced toxicity with other toxins. [0004] Fumonisins are seco...

AI Technical Summary

Problems solved by technology

Although there are currently detection kits for zearalenone and fumonisin B1, each detection kit can only detect one toxin, and cannot detect two toxins at the same time. The operation is troublesome, uneconomical and environmentally friendly. , has seriously affected the detection of mycotoxins in actual work. The present invention will fundamentally provide a fast and simple detection method, and provide a fast one-step detection method for entry-exit quarantine, customs, production enterprises, supervision departments and other units. Mycotoxin and Fumonisin B1

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