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Tissue culture method of president clematis

A tissue culture, presidential technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problems of insufficiency, high cost, slow division speed, etc., to improve the propagation speed of seedlings and ensure market supply demand. , to achieve the effect of seedling factory

Active Publication Date: 2013-03-06
上海上房园艺有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is an excellent new variety obtained through artificial breeding, because its ramet speed is slow, the cutting propagation rate is low, and the variety is easy to degenerate, which cannot meet the large demand of the market
Some companies directly import bare-root seedlings from abroad for sale, which has high costs and low profits. Therefore, how to increase production and reduce cultivation costs is a problem that needs to be solved urgently

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for tissue cultivating 'President' Clematis, the method comprises the following steps:

[0025] (1) Acquisition of sterile raw materials: After picking the young shoots of Clematis 'President', rinse them with tap water for 2 hours, soak them in 75 wt% ethanol for 25 seconds on the ultra-clean workbench, soak them in 1w‰ of mercury chloride for 10 minutes, and use After rinsing with sterile water for 5 times, blot the surface moisture, take the base of the shoots, cut them into 1cm lengths and inoculate them on the bud induction medium. The composition of the bud induction medium is MS+6-BA1.0mg / L+NAA0. 1mg / L;

[0026] (2) Differentiation and proliferation of buds: 5 weeks after the buds were inoculated in the bud induction medium, the base of the buds began to expand and yellow-green protrusions appeared. After 3 weeks of continuous cultivation, obvious callus tissue was visible. The callus with lower buds is inoculated on the adventitious bud proliferation m...

Embodiment 2

[0032] A method for tissue cultivating 'President' Clematis, the method comprises the following steps:

[0033] (1) Obtain sterile raw materials: pick the young shoots of Clematis 'President' and wash them with tap water for 2 hours, then soak them in 75wt% ethanol for 35s and 1w‰ of mercuric acid for 15 minutes on the ultra-clean workbench, and then use After rinsing with sterile water for 6 times, blot the surface moisture, take the base of the shoots, cut them into 1cm lengths and inoculate them on the bud induction medium. The composition of the bud induction medium is MS+6-BA5.0mg / L+NAA0. 5mg / L;

[0034] (2) Differentiation and proliferation of buds: 5 weeks after the buds were inoculated in the bud induction medium, the base of the buds began to expand and yellow-green protrusions appeared. After 3 weeks of continuous cultivation, obvious callus tissue was visible. The callus with lower buds was inoculated on the adventitious bud proliferation medium. The composition of...

Embodiment 3

[0040] A method for tissue cultivating 'President' Clematis, the method comprises the following steps:

[0041] (1) Obtain sterile raw materials: pick the young shoots of clematis 'President' and rinse them with tap water for 2 hours, then soak them in 75wt% ethanol for 30s and 1w‰ of mercuric chloride for 15mins on the ultra-clean workbench, and use After rinsing with sterile water for 6 times, blot the surface moisture, take the base of the shoots, cut them into 1cm lengths and inoculate them on the bud induction medium. The composition of the bud induction medium is MS+6-BA3.0mg / L+NAA0. 3mg / L;

[0042](2) Differentiation and proliferation of buds: 5 weeks after the buds were inoculated in the bud induction medium, the base of the buds began to expand and yellow-green protrusions appeared. After 3 weeks of continuous cultivation, obvious callus tissue was visible. The callus with lower buds was inoculated on the adventitious bud proliferation medium. The composition of the ...

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PUM

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Abstract

The invention relates to a tissue culture method of president clematis. Tender shoots of 'president' clematis are selected as a raw material; and a clematis 'president' plant is acquired through steps of an aseptic processing, a differentiation and propagation of tender shoots, a strong seedling culture of adventitious buds, a rooting culture of a root, a plant hardening seedling and transplanting, etc. Compared with a prior art, the invention employs tissue culture to increase a breeding speed of nursery stock and regularity of the seedlings, to better maintain original female parent properties, and to realize factory seedling growing, so as to guarantee market supply demand.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for tissue culture 'President' clematis. Background technique [0002] Clematis is a plant of the genus Clematis in the family Ranunculaceae. It is native to China and widely distributed in the northern hemisphere. There are more than 200 original species. After years of artificial hybridization, a large number of horticultural varieties have emerged. Different varieties can be opened from May to November. The spectacular flowering scene is welcomed by the world, and it is currently a very popular garden vine material in China. [0003] Clematis 'President' is a gardening variety, and the woody vines are about 1-2 meters long. Stems brown or purple, single leaves, often opposite; flowers solitary, purple. The flowering period is from June to September. It is an excellent new variety obtained through artificial breeding. Because of its slow ramet speed, low cutting pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 陈建华黄建荣沈勤
Owner 上海上房园艺有限公司
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