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Immunochromatographic test paper for detecting diethylstilbestrol and preparation method thereof

A technology of immunochromatography test strips and diethylstilbestrol, which is applied in biological testing, measuring devices, analytical materials, etc., and can solve problems such as insufficient magnetic resonance signals, easy aggregation of magnetic beads, and long color development time

Inactive Publication Date: 2011-11-23
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these preparation and modification methods are often cumbersome and complicated, and the obtained superparamagnetic composite particles cannot meet the requirements of LFIAs in terms of size, biocompatibility, saturation magnetic strength, external magnetic field response speed, stability, and labeling efficiency. The size is mostly between 200 and 300nm. Due to the large size of the magnetic beads, the swimming time on the test paper is slow and the color development time is long; and the particles that are too small cannot provide sufficient magnetic resonance signals; in addition, there are biological Unstable compatibility and easy aggregation of magnetic beads; these shortcomings limit the application of superparamagnetic composite particles in LFIAs

Method used

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  • Immunochromatographic test paper for detecting diethylstilbestrol and preparation method thereof
  • Immunochromatographic test paper for detecting diethylstilbestrol and preparation method thereof
  • Immunochromatographic test paper for detecting diethylstilbestrol and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1, Preparation of magnetically labeled rapid detection test paper for diethylstilbestrol residues

[0090] (1) Preparation of antibody to diethylstilbestrol labeled with superparamagnetic composite particles

[0091] The particle size is 100nm, the magnetic saturation intensity is 40emu / g, the corresponding external magnetic field response speed is 20 seconds, and the superparamagnetic composite particle (superparamagnetic Fe 3 o 4 Nanoparticles) (purchased from Shenzhen Thales Technology Co., Ltd., catalog number MP-2) labeled diethylstilbestrol antibody.

[0092] The specific method is:

[0093] 1) Take 2.5mg of the above-mentioned magnetic particles, wash them with 0.1M MES buffer (weigh 1.066g MES, 0.45g NaCl and dissolve them in 50ml pure water, adjust the pH to 4.7), separate and enrich them with a 0.4T magnetic rack, and use 1ml of MES buffer with a concentration of 0.1M and a pH value of 4.7 was resuspended, and 0.96mg (final concentration of 5mM) of ...

Embodiment 2

[0107] Embodiment 2, the detection of the immunochromatographic test paper sensitivity, cross-reactivity and accuracy of diethylstilbestrol residue

[0108] 1. Determination of sensitivity

[0109] Weigh an appropriate amount of diethylstilbestrol (Beijing Hengyuan Qitian Chemical Technology Research Institute, catalog number: 30216CDCT-C12607000), and prepare a 100ug / ml standard stock solution with methanol. Dilute the standard solution prepared as 0, 0.005, 0.01, 0.05, 0.1, 1, 5, 10 ug / L with 0.02M PBS (pH=7.4), add respectively in the immunochromatographic test paper for detecting diethylstilbestrol obtained by Example 1 , and read using a superparamagnetic resonance detector MAR (MagnaBioSciences, 8094-101-01&8094-101-02). Detection steps: Return the sample to be tested to room temperature (25°C) before detection, use a precise pipette to take 100 μl of the sample to be tested and drop it vertically and slowly into the sample loading end of the magnetic test strip, and th...

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Abstract

The invention discloses an immunochromatographic test paper for detecting diethylstilbestrol and a preparation method thereof. The immunochromatographic test paper for detecting diethylstilbestrol provided in the invention comprises a sample pad containing superparamagnetic composite particle marked antibodies to diethylstilbestrol, a cellulose nitrate membrane connected with one end of the pad and a water absorbing pad connected with another end of the cellulose nitrate membrane, wherein, the cellulose nitrate membrane is coated with a detection line and a quality control line which are mutually separated, the detection line contains diethylstilbestrol antigen, and the quality control line contains antiantibodies capable of specific binding to the antibodies to diethylstilbestrol. Experiments in the invention indicate that the test paper provided in the invention has the characteristics of high sensitivity, strong specificity, rapidness, simpleness and capacity of realizing objective detection in detecting diethylstilbestrol.

Description

technical field [0001] The invention relates to a detection reagent for diethylstilbestrol residues, in particular to an immunochromatographic test paper for detecting diethylstilbestrol and a preparation method thereof. Background technique [0002] Diethylstilbestrol (DES) is a synthetic non-steroidal estrogen, because it can promote protein synthesis, accelerate weight gain and bone calcification, and reduce feed consumption, it is used as an animal growth promoter in livestock and poultry production and Aquaculture etc. However, many scientific experiments have confirmed that diethylstilbestrol is a carcinogen, which is harmful to human and animal health. Therefore, many countries have stipulated that diethylstilbestrol is prohibited in livestock and poultry and aquaculture. my country is a big country in the production, consumption and export of aquatic products, and diethylstilbestrol is one of the main monitoring drug residues in aquatic products in recent years. At...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/531
Inventor 马岚卢体康秦智锋袁航吴峰
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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