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Novel immunologic detection method

An immunological detection method and immunological detection technology, applied in the direction of biological testing, material inspection products, etc., can solve the problems of inaccurate quantification, cumbersome operation, long time, low sensitivity, etc., and achieve the effects of saving dosage, improving efficiency and accelerating response

Inactive Publication Date: 2011-11-16
SINNOWA MEDICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current immunoassay methods are basically based on methods such as microwell plates, magnetic beads, and immunochromatographic diffusion. The main defects of these detection methods are: individual items must be detected step by step, and the operation is cumbersome and takes a long time, such as: enzyme immunoassay, chemiluminescence, etc. detection; or simple and fast but low sensitivity, unable to accurately quantify, such as immunoassay card or strip technology

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0007] The method is illustrated by taking the detection of 5 different antigens as an example. Put 5 immune probes labeled with 5 corresponding antibodies into the cuvette containing the samples to be tested at the same time; then place the cuvette in the thermostat and rotate slowly to enhance the combination of antigen and antibody; after the reaction is over, pour out the sample solution; Take out the immunoprobes after washing and place them separately in different detection cups; add the labeled antibody according to the target antigen to be detected, and slowly rotate the reaction in the thermostat; pour off the reaction liquid and wash; add the chromogenic agent and develop the color; observe Color development (enzyme labeling method). Or add a luminescent reagent; detect luminescence (chemiluminescence).

Embodiment 2

[0009] 5 different antibodies were tested. Put 5 immune probes labeled with 5 different antigens into the reaction cup containing the sample to be tested at the same time, then place the reaction cup in a thermostat and rotate slowly to enhance the combination of antigen and antibody; after the reaction, put the immunoprobe in the original cup After the needle is washed, add the labeled anti-antibody, and slowly rotate the reaction in a thermostat; after the reaction is completed, take out the immune probe after washing and place it in different detection cups, add the chromogenic reagent to each reaction cup, and detect or observe separately after color development As a result, the detection ends.

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Abstract

The invention relates to a novel immunologic detection method. The method is characterized in that: known antigens used for detection or known antibodies used for detection are respectively labeled to a newly-designed immunoprobe; then a plurality of the labeled immunoprobes are placed into a reaction cup filled with a sample requiring detection or a reaction vessel filled with the sample requiring detection during detecting, such that a plurality of immunobinding reactions can be respectively and simultaneously performed in the same reaction system, and a plurality of items of one sample can be simultaneously detected without sample allocating.

Description

1. Technical field [0001] A novel immunological detection method belongs to the technical field of immunological detection methods. 2. Background technology [0002] Immunological detection is a very important detection method for the diagnosis of clinical infectious diseases, tumors, hormones and other diseases, and is also widely used in many fields such as agriculture, pharmaceuticals, food, environment and life sciences. Immunological testing often requires multiple, even dozens of immune items to be checked on the same sample. The current immunoassay methods are basically based on methods such as microwell plates, magnetic beads, and immunochromatographic diffusion. The main defects of these detection methods are: individual items must be detected step by step, and the operation is cumbersome and takes a long time, such as: enzyme immunoassay, chemiluminescence, etc. detection; or simple and fast but low sensitivity, which cannot be accurately quantified, such as immun...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/52
Inventor 徐新
Owner SINNOWA MEDICAL SCI & TECH
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