Preparation method for producing lipase by solid fermentation of waste residue

Abstract

The invention provides a preparation method for producing lipase by solid fermentation of waste residue and belongs to the field of microbial fermentation. The method comprises: inoculating Aspergillus niger RF2-5 into a fermentation culture medium, performing solid fermentation in the presence of the air and at a temperature of 35 to 42 DEG C and an ambient relative humidity of 75 to 80 percent,and culturing for three days till the lipase production peak; leaching cultured material in 100 millimoles of phosphoric acid buffer solution with a pH value of 7.0, centrifuging, and collecting supernate which is solution of the lipase; and testing the activity of the lipase by a titration process, wherein the activity of the lipase reaches 32.3 unit per gram of dry solid. The lipase prepared bythe method has the advantages that: the raw materials are cheap; the yield is high; the fermentation period is short; the process is simple; and the industrial development value is high. In addition,the invention provides a new approach for treating high-concentration lipa waste water.

Description

technical field [0001] The invention relates to a preparation method for producing lipohydrolase by solid-state fermentation of waste residue, more precisely, relates to a method for producing lipohydrolase by solid-state fermentation of waste residue by using Aspergillus niger RFW-5 as a strain. Background technique [0002] Sewage from slaughterhouses, dairy processing plants and the catering industry contains a large amount of non-biodegradable oils and fats. A high concentration of oil will cause a series of problems in the subsequent treatment, such as reducing mass transfer efficiency, multiplying filamentous bacteria and causing difficulty in sedimentation, floating sludge, clogging pipe network and generating malodorous gas. [0003] Lipohydrolase is an enzyme preparation whose main function is to hydrolyze fat and convert fat into glycerol and fatty acids. A large number of scholars (such as Denise M.G. Freire, Magali C. Cammarota, Daniela R. Rosa, etc. in Brazil) ...

AI Technical Summary

Problems solved by technology

[0004] At present, most commercial lipohydrolytic enzymes are obtained by liquid submerged fermentation. Although the purity is high, the high price limits its wide application.