Method for screening transgenic radish in field by using kanamycin leaf smearing method

Abstract

The invention provides a method for screening transgenic radish in field by using a kanamycin leaf smearing method. The experimental material is transgenic radish of neomycin phosphotransferase II (NPT II)-labeled genes, the optimal concentration of the field screening of Kan leaf smearing is 1,000mg / L, and the observation time is preferably 12 days; and verified by polymerase chain reaction (PCR) detection, the positive detection rate of Kan screened resistance seedlings reaches 93.8 percent. According to the invention, the simple and convenient method for screening a large amount of transgenic radish in field is researched and established, and has important value on breading of the transgenic radish material.

Description

technical field [0001] The invention belongs to the field of genetically modified crop breeding, and in particular relates to a method for screening transgenic radishes in the field using kanamycin leaf smearing method Background technique [0002] Radish (Raphanus sativus L.), also known as radish and calabash, belongs to the genus Radish of Brassicaceae. The root is the main carnivorous organ and is rich in nutrition. It is cultivated all over the world. [0003] The genetic modification of radish is still in its infancy. For the screening and identification of transformed materials, using traditional molecular detection methods is not only heavy workload, high cost, but also difficult to operate. At the same time, the genetic rules of the progeny of the obtained plants need to be studied, which requires a lot of screening and identification of transgenic progeny. Therefore, more and more attention has been paid to the rapid detection method of transgenic plants using con...

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Problems solved by technology

[0004] The method for rapidly screening transgenic plants with kanamycin in the field has been reported in transgenic cotton, soybean, tomato, rice, and wheat, but has not been reported in the screening of transgenic radish plants.