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Method for synthesizing medicament L-dopa by enzyme catalysis

A technique for synthesizing the drug, dopa, applied in the field of enzyme-catalyzed synthesis of the drug L-dopa, which achieves the effects of cost reduction, mild reaction conditions, and a single component

Inactive Publication Date: 2011-10-12
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no reference to the application of immobilized tyrosinase, especially carrier-free immobilized tyrosinase, for the production of L-dopa.

Method used

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  • Method for synthesizing medicament L-dopa by enzyme catalysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Pack the carrier-free immobilized tyrosinase into a column, let the mixture of L-tyrosine and L-ascorbic acid pass through the column with the carrier-free immobilized tyrosinase, and the effluent product mixture You can get L-dopa. The conversion rate of this method can reach more than 25% after 0.5h.

Embodiment 2

[0039] Add the mixture of carrier-free immobilized tyrosinase, L-tyrosine and L-ascorbic acid into a batch reactor for reaction for a period of time, remove the carrier-free immobilized tyrosinase, and obtain L-dopa . This method reacts for 2 hours, and the conversion rate of more than 60% can be obtained. Carrier-free immobilized tyrosinase can be centrifuged at 6000r / min at room temperature to remove most of it first, and then filter to remove the remaining small part of enzyme powder.

Embodiment 3

[0041]Add carrier-free immobilized tyrosinase into a continuous reactor with a mixture of L-tyrosine and L-ascorbic acid, magnetically stir the reaction, and continuously export the generated product L-dopa, and use the same Introduce the fresh L-tyrosine and L-ascorbic acid reaction solution at a high speed. After 1 hour, the conversion rate can reach more than 50%.

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Abstract

The invention belongs to the technical field of preparation of a medicament L-dopa. In order to solve the technical problem that the medicament L-dopa is difficultly synthesized by biological catalysis in the prior art, the invention provides a method for synthesizing a medicament L-dopa by enzyme catalysis. In the method, L-tyrosine is used as a raw material, and the L-dopa is generated by catalysis of carrier-free immobilized tyrosinase.

Description

【Technical field】 [0001] The invention belongs to a method for synthesizing drug L-dopa with enzyme catalysis, in particular to a synthesis method for catalyzing L-dopa with a carrier-free immobilized tyrosinase. 【Background technique】 [0002] As a kind of biocatalyst, enzyme has been widely used in food production and testing, environmental protection technology, biopharmaceutical and other fields in recent years. However, enzymes are sensitive to the environment and difficult to recover after reaction, which limits the development and application of enzyme preparation products. In this case, immobilized enzyme technology came into being. Enzyme immobilization is a kind of technology that binds or confines enzymes in a certain area with solid materials, but can still perform its unique catalytic reactions, and can be recycled and reused. Compared with free enzymes, immobilized enzymes overcome the shortcomings of free enzymes while maintaining their high-efficiency, spec...

Claims

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Application Information

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IPC IPC(8): C12P13/22
Inventor 杨缜徐大友
Owner SHENZHEN UNIV
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