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Method for identifying single-spore bacteria strain of straw mushroom Pingyou NO.1 by using co-dominant marker

A technology of co-dominant markers and bacterial strains, applied in biochemical equipment and methods, microbial measurement/inspection, etc., to achieve high sensitivity, easy judgment, and great application value

Inactive Publication Date: 2012-09-19
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

However, straw mushroom is considered to be a homophyletic fungus with multinucleated mycelium cytoplasm and no lock-like association, so it is difficult to distinguish the homokaryotic and heterokaryotic hyphae or parents and hybrids of straw mushroom in physiological form, which hinders the genetic breeding of straw mushroom Research

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  • Method for identifying single-spore bacteria strain of straw mushroom Pingyou NO.1 by using co-dominant marker

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Embodiment 1

[0021] Embodiment 1: A kind of method of using co-dominant markers to identify the single spore strain of the clover mushroom Pingyou No. 1

[0022] A method utilizing co-dominant markers to identify Pingyou No. 1 single-spore strain of clover mushrooms, comprising the following steps:

[0023] 1. Genome sequencing of two monospore strains of Straw Mushroom Pingyou No. 1

[0024] The straw mushroom Pingyou No. 1 strain was purchased from Pingnan County, Fujian Province. After the fruiting test, the monospores were ejected to obtain the single-spore strains, and two single-spore strains were obtained through the paired fruiting test again. The total DNA of two monosporus strains was extracted, and Shenzhen Huada Gene Technology Co., Ltd. was entrusted to conduct whole genome sequencing.

[0025] 2. Co-dominant marker primer design

[0026] Homology analysis was carried out on the sequenced genomes of the 2 species of V. volvulus Pingyou No. 1 single spore strain, and the ...

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Abstract

The invention provides a method for identifying a single-spore bacteria strain of a straw mushroom Pingyou NO.1 by using a co-dominant marker, which comprises the following steps of: carrying out sequence analysis on the genomes of two single-spores of the straw mushroom Pingyou NO.1, designing co-dominant marker primers of the two signal-pores, cultivating and collecting mycelium, extracting theDNA of the genomes, detecting and establishing the co-dominant molecule marker, and detecting co-dominant marker PCR products, wherein the co-dominant marker primers are Pingyou F5'-CAATCACTCTTCCGAGGCTC-3' and Pingyou R5'-GGATACGCATGTTAAAGTGG-3'. The method for identifying the single-spore bacteria strain of the straw mushroom Pingyou NO.1 by using the co-dominant marker solves the identificationproblem of the single-spore bacteria strain of the straw mushroom and has the advantages of high sensitivity, short detecting time, reliable detection results, easiness in judgment, good repeatability and the like.

Description

technical field [0001] The invention relates to a method for co-dominant markers of straw mushrooms, more specifically to a method for identifying the monospore strain No. 1, Pingyou No. 1, of straw mushrooms by using co-dominant markers, and belongs to the field of biotechnology. Background technique [0002] straw mushroom[ Volvariella volvacea (Bull.:Fr.) Sing.], also known as straw mushroom, orchid mushroom, stalk mushroom, sesame mushroom, home-grown mushroom, Nanhua mushroom, grass mushroom, delicious bract, foot mushroom, in European and American countries it is called straw mushroom, Chinese mushrooms. Straw mushroom originated in Nanhua Temple in Shaoguan, Guangdong. It is an important tropical and subtropical mushroom and the third largest cultivated edible fungus in the world. It is mainly distributed in Guangdong, Guangxi, Fujian, Hunan, Taiwan, Hainan, etc. in China. In recent years, due to the adoption of technical facility cultivation and factory cultiva...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04
Inventor 刘新锐谢宝贵邓优锦江玉姬朱坚章小寅刘朋虎
Owner FUJIAN AGRI & FORESTRY UNIV
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