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A strain of endometrial adenocarcinoma cell lines

An endometrial, adenocarcinoma-like technology, applied in the field of human endometrioid adenocarcinoma cells, can solve problems such as limited cell lines, and achieve the effect of broad application prospects

Inactive Publication Date: 2012-10-24
廖秦平 +5
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the phenotype diversity and tumor heterogeneity of endometrial cancer, the established cell lines are still very limited

Method used

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  • A strain of endometrial adenocarcinoma cell lines
  • A strain of endometrial adenocarcinoma cell lines
  • A strain of endometrial adenocarcinoma cell lines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. Establishment and identification of human endometrioid adenocarcinoma cell LHY-821 CGMCC №.4066

[0022] 1. Establishment of human endometrioid adenocarcinoma cell line LHY-821 CGMCC №.4066

[0023] The tumors of endometrial cancer patients were clinically collected for primary cell culture of endometrial cancer. After digesting and culturing the tumors of 14 endometrial cancer patients, an endometrial cancer cell line capable of continuous passage was found, and it has been passed down to 82 generations. The patient was a moderately differentiated endometrioid adenocarcinoma, and the clinical records were as follows:

[0024] Patient medical records: female 50y

[0025] Freezing + paraffin:

[0026] (Right ovary) There was no significant change in the oviduct of moderately differentiated endometrioid adenocarcinoma, and no lymph node metastasis.

[0027] ER++, PR+++, EMA+, VIM+, P53-, CA125-.

[0028] Total hysterectomy and partial vaginal resection spec...

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Abstract

The invention discloses a strain of endometrial adenocarcinoma cell lines, wherein a name of the endometrial adenocarcinoma cell lines is LHY-821, and a preservation register number of the endometrial adenocarcinoma cell lines is CGMCC No.4066. The LHY-821 cell lines has advantages of uniform cell morphology, good cell growth, strong adhesive ability, doubling time of 44-48 hours and stable biological characteristics and genetic property. The LHY-821 cell lines can be applicable for research on biology of tumor cells, molecular biology, gene and epigenetics in vitro and in vivo, and on therapeutic effects of new antitumor drugs in a preclinical phase, and has a wide application prospect.

Description

technical field [0001] The present invention relates to a strain of human endometrial adenocarcinoma cell line. Background technique [0002] Endometrial cancer is one of the three major tumors of the female reproductive tract, and its incidence has been increasing in recent years. Women's prolongation of life expectancy and increasing incidence of morbidity suggest that revealing its pathogenic mechanism is an urgent problem to be solved. The isolation and purification of human endometrial adenocarcinoma cells, as well as the study of their characteristics, can contribute to the study of tumor mechanism and the study of chemotherapy resistance mechanism. Compared with the phenotype diversity and tumor heterogeneity of endometrial cancer, the established cell lines are still very limited. Such as Ishikawa, RL95-2, HEC-1A and KLE etc. Therefore, it is necessary for us to establish a relatively complete library of endometrial cancer cell lines to provide a suitable in vitro...

Claims

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Application Information

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IPC IPC(8): C12N5/09
Inventor 廖秦平胡倩于丽张岩陈锐谢娅
Owner 廖秦平
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