Method for obtaining influenza virus nucleic acid in influenza sample and special primer thereof

Abstract

The invention discloses a method for obtaining nucleic acid of influenza virus in an influenza sample and a special primer thereof. A primer pair for amplifying the nucleic acid of the influenza virus consists of a primer as shown in a sequence 9 in a sequence table and a primer as shown in a sequence 10 in the sequence table. The method for obtaining influenza viral genome DNA (deoxyribonucleic acid) comprises the following steps: based on the total RNA (ribonucleic acid) of a sample infected by influenza virus as a template, carrying out RT-PCR (reverse transcription-polymerase chain reaction) amplification with the primer pair so as to obtain a RT-PCR amplification product, namely the nucleic acid of the influenza virus. Through the method in the patent, virus separation is not carried out and the genome of the influenza virus is amplified directly from a nucleic acid extractive of a clinic sample, thereby greatly simplifying the program of influenza virus gene research and saving experiment cost. Based on the method, the primer is amplified through designing the segments of the influenza virus, so that all the segments of the influenza virus can be directly and successfully amplified.

Description

technical field [0001] The invention relates to a method for obtaining influenza virus nucleic acid in an influenza sample and special primers thereof in the field of biotechnology. Background technique [0002] In history, there are many kinds of plagues that have brought a large number of deaths and serious disasters to human beings. In the past hundred years, due to the development and progress of medical science, most of the plagues have been controlled, except for influenza, which humans have not found an effective way and method to subdue it. [0003] Influenza viruses belong to the Orthomyxoviridae family and include three genera: A, B, and C. Because of its strong infectivity, influenza virus is considered to be an important risk factor affecting public health safety, among which influenza A is the most serious. In particular, the outbreak of a new influenza A virus (swine H1N1), which was discovered in Mexico in March 2009 and caused widespread worldwide spread, h...

AI Technical Summary

Problems solved by technology

However, in laboratory monitoring of influenza, if the method of virus isolation using cells is used for laboratory monitoring, it will not only take a long time but also require higher experimental conditions for new influenza variants.

These factors contribute to the increased cycle time for influenza virus isolation

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