Application of rubbish compost compound bactericide in aspect of improving mineral nutrition in turfgrass

A compound bacterial agent and mineral nutrition technology, applied in bacteria and other directions, can solve the problems of complexity, composting bacteria symbiosis and ambiguity of mutual influence relationship.

Inactive Publication Date: 2013-06-12
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the research process of compound microbial agents, the identification and classification of microbial species in compost, the symbiosis and mutual influence relationship between compost strains are ambiguous and complex, and the application of compost microbial agents to lawn plants is of great importance. Requirements and effects of mineral nutrition in the body are rarely reported

Method used

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  • Application of rubbish compost compound bactericide in aspect of improving mineral nutrition in turfgrass
  • Application of rubbish compost compound bactericide in aspect of improving mineral nutrition in turfgrass
  • Application of rubbish compost compound bactericide in aspect of improving mineral nutrition in turfgrass

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Experimental program
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Effect test

Embodiment 1

[0068] Strain screening method

[0069] Enrichment: Weigh 10g of the collected compost sample and place it in a sterile Erlenmeyer flask, add 100ml of sterile water and glass beads and shake evenly, then take 10ml of the suspension in a Erlenmeyer flask filled with 100ml of enrichment medium, respectively Cultivate on a shaker at a suitable temperature for 3-5 days. Primary screening: After diluting the enriched culture solution in a concentration gradient, spread it on the separation and screening medium, culture it upside down at a constant temperature, observe the size of the transparent circle, and select the strain with a larger ratio of transparent circle / colony diameter for secondary screening . Re-screening: drawing of growth curve: Inoculate the strains obtained from the primary screening into the corresponding shaker flask culture medium, and cultivate them under the conditions of suitable temperature and shaker speed. Take the OD value as the vertical axis and the ...

Embodiment 2

[0073] Soilless cultivation of plants: lawn plant seeds treated with 1% H 2 o 2 The solution was sterilized for 3-5min, then rinsed with distilled water, put the seeds into a centrifuge tube, and placed in saturated CaSO 4 After soaking in the solution for 4 hours, add a little CaSO 4 solution, wherein a layer of filter paper is placed on it, and the seeds soaked for 4 hours are placed on the filter paper, and 100 turf plant seeds with full seeds and the same size after soaking are selected, rinsed with distilled water and sown in it. In the test, each treatment is set to 3 Repeat for three times, adding distilled water in the morning and evening to replenish the water lost by evaporation.

[0074] Application of compound microbial agent: Treatment 1 adds the original agent, referred to as CM 1 ; Treatment 2 added 100 times liquid, referred to as CM 2 ; Treatment 3 added 200 times liquid, referred to as CM 3 ; Treatment 4 added 300 times liquid, referred to as CM 4 ; Add...

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Abstract

The invention discloses an application of a house rubbish compound bactericide in the aspect of improving the mineral nutrition requirement in a lawn plant body. Three strains namely, bacillus subtilis, actinomycete and saccharomycete are diluted by 100-200 times so as to form the compound bactericide, wherein the volume ratio of bacillus subtilis to actinomycete to saccharomycete is 1: 1: 1. After the compost compound microorganism bactericide is sprayed, the variation tendencies of a large amount of elements (Na, K, Ca and Mg) are the same basically along with the increase of dilution multiple of the bactericide, and the influences of the compost compound microorganism bactericide on lawn plant growth due to host plant varieties and environment factors are obviously different, which is beneficial to absorption and utilization of the lawn plants on mineral elements.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to the preparation of a high-efficiency composting compound bacterial agent by using beneficial bacteria extracted from urban domestic garbage composting as raw materials. More specifically, it uses microbial inoculum to study the impact and application on the initial growth of ryegrass and tall fescue. Background technique [0002] Composting is a biochemical process that uses microorganisms (bacteria, actinomycetes, fungi, etc.) widely present in nature or commercial strains to controlly promote the transformation of biodegradable organic matter into stable humus (humic substance, HS). . This process is also part of the ecological process on the earth's surface, which participates in the cycle of matter and energy on the earth's surface. [0003] Under artificial control conditions, the organic matter in solid waste can be converted into organic fertilizer (compos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
Inventor 多立安赵树兰王晶晶
Owner TIANJIN NORMAL UNIVERSITY
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