TiO2 (titanium dioxide) nanowire biosensor chip and system for fast detecting Enteropathogenetic Escherichia coli (EPEC)

Abstract

The invention discloses a TiO2 (titanium dioxide) nanowire biosensor chip and system for fast detecting Enteropathogenetic Escherichia coli (EPEC). The TiO2 nanowire biosensor chip is that TiO2 nanowires are connected between two parallel metal electrodes; the TiO2 nanowires are in a coaxial layered nano-structure; and the surface of the TiO2 nanowires is bonded with hydroxyl. A sample feeding container and a sample discharging container of the biosensor of a system for fast detecting EPEC are communicated by a microfluidic channel; the TiO2 nanowires in the TiO2 nanowire biosensor chip are disposed in the microfluidic channel; the two parallel metal electrodes in the TiO2 nanowire biosensor chip are respectively disposed at two sides of the microfluidic channel; and the TiO2 nanowires are disposed in an alternating magnetic field; a first peristaltic pump of the system is communicated with the sample feeding container by a bacteria feeding tube; a second peristaltic pump is communicated with the sample feeding container by a cleaning solution feeding tube; and an impedance detection circuit is respectively and electrically connected to the two metal electrodes, the first peristaltic pump and the second peristaltic pump. In the invention, complicated pre-processes for bacteria samples are not needed; and the bacteria detection time is shortened.

Description

technical field [0001] The present invention relates to a TiO for rapid and sensitive detection of pathogenic Escherichia coli 2 Nanowire biosensor chip detection system. Background technique [0002] Traditional bacterial detection methods such as cell culture and fluorescence testing not only have a long detection cycle but also consume a lot of manpower and material resources, which are difficult to meet the current requirements for food safety testing at home and abroad. At present, compared with traditional methods, commonly used detection methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) can effectively shorten the analysis time, but Its detection time is still as long as five to six hours, and it cannot realize real-time detection of bacteria. Therefore, realizing rapid and real-time detection of bacteria to ensure the safety of people's daily life has become an important problem that needs to be solved urgently. [0003]...

AI Technical Summary

Problems solved by technology

[0002] Traditional bacterial detection methods such as cell culture and fluorescence testing not only have a long detection cycle but also consume a lot of manpower and material resources, which are difficult to meet the current requirements for food safety detection at home and abroad

At present, compared with traditional methods, commonly used detection methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) can effectively shorten the analysis time, but Its detection time is still as long as five to six hours, and it cannot achieve real-time detection of bacteria

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