Recombinant ubiquitin ligase PTB-U-box fusion gene and expression vector and application thereof
A ubiquitin ligase, ptb-u-box technology, applied in gene therapy, drug combination, genetic engineering and other directions, to achieve the effect of reducing cell proliferation and invasion ability
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[0035] The present invention utilizes the PTB domain (Phosphotyrosine Binding domain) of linker molecule IRS-1 (Insulin Receptor Substrate 1) to be able to recognize and bind to the characteristics of insulin-like growth factor receptor (Insulin-like Growth Factor Receptor 1, IGF-1R) and CHIP To construct a recombinant ubiquitin ligase PTB-U-box that can target and degrade the oncogenic-related protein IGF-1R. The constructed fusion gene PTB-U-box is to clone the PTB domain gene of IRS-1 and the U-box domain gene of CHIP with ubiquitin ligase activity respectively, and use recombinant PCR to fuse; wherein, the PTB domain is responsible for For the targeted binding of IGF-1R, U-box is responsible for connecting ubiquitin molecules to substrates to promote ubiquitination and degradation of substrates. In the following, the present invention will be further described in detail in conjunction with the construction of the fusion gene PTB-U-box, eukaryotic expression vector and reco...
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