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Culture solution increasing content of peptides contained in sprouted soybeans

A technology for germinating soybeans and culture solution, which is applied in fertilizer mixtures, fertilization devices, applications, etc., can solve the problems of insufficient stimulation of soybean nutrition, and achieve the effects of easy industrial production, easy operation, and scientific and reasonable formula.

Inactive Publication Date: 2011-09-07
常州五星禾绿蔬菜食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the above-mentioned defects, the technical problem to be solved in the present invention is to provide a soybean germination culture solution for the problem that the nutrition in the soybean cannot be fully stimulated in the existing soybean germination technology.

Method used

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  • Culture solution increasing content of peptides contained in sprouted soybeans
  • Culture solution increasing content of peptides contained in sprouted soybeans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] In the culture medium, the concentration of gibberellin was 55×10 -6 mol / L, the concentration of calcium chloride is 6×10 -3 The concentration of mol / L and manganese sulfate is 10×10 -3 mol / L.

[0017] Select mature, plump and undamaged soybeans, immerse them in a mass ratio of 1% sodium hypochlorite solution for disinfection for 30 minutes at a ratio of 1:5, and then rinse them with water; place the disinfected soybeans in a pH 5.2 citric acid-sodium citrate buffer solution at a ratio of 1:5 Soak in (CBS) at a temperature of 25°C for 6 hours, then transfer to the above-mentioned culture solution for infiltration and cultivation for 6 days, during which time the culture solution is sprayed on the germinated soybeans every 12 hours, the culture temperature is 15-29°C, and the culture environment is kept moist. . The culture medium used was taken out after 6 days of germination, and the peptide content in germinated soybeans was 102.47 mg·g -1 .

Embodiment 2

[0019] In the culture medium, the concentration of gibberellin is 72×10 -6 mol / L, the concentration of calcium chloride is 7.5×10 -3 The concentration of mol / L and manganese sulfate is 12×10 -3 mol / L.

[0020] Select mature, plump and undamaged soybeans, immerse them in a mass ratio of 1% sodium hypochlorite solution for disinfection for 30 minutes at a ratio of 1:5, and then rinse them with water; place the disinfected soybeans in a pH 5.2 citric acid-sodium citrate buffer solution at a ratio of 1:5 Soak in (CBS) at a temperature of 25°C for 6 hours, then transfer to the above-mentioned culture solution for infiltration and cultivation for 6 days, during which time the culture solution is sprayed on the germinated soybeans every 12 hours, the culture temperature is 15-29°C, and the culture environment is kept moist. . The culture medium used was taken out after 6 days of germination, and the peptide content in germinated soybeans was 122.49 mg·g -1 .

Embodiment 3

[0022] In the culture medium, the concentration of gibberellin was 45×10 -6 mol / L, the concentration of calcium chloride is 12×10 -3 The concentration of mol / L and manganese sulfate is 10×10 -3 mol / L.

[0023] Select mature, plump and undamaged soybeans, immerse them in a mass ratio of 1% sodium hypochlorite solution for disinfection for 30 minutes at a ratio of 1:5, and then rinse them with water; place the disinfected soybeans in a pH 5.2 citric acid-sodium citrate buffer solution at a ratio of 1:5 Soak in (CBS) at a temperature of 25°C for 6 hours, then transfer to the above-mentioned culture solution for infiltration and cultivation for 6 days, during which time the culture solution is sprayed on the germinated soybeans every 12 hours, the culture temperature is 15-29°C, and the culture environment is kept moist. . The culture medium used was taken out after 6 days of germination, and the peptide content in germinated soybeans was 142.13 mg·g -1 .

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Abstract

The invention relates to a culture solution increasing the content of peptides contained in sprouted soybeans and an application method thereof. A solution comprises gibberellin, calcium chloride and manganese sulfate, wherein the concentration of the gibberellin is 40*10<-6>-80*10<-6> mol / L, the concentration of the calcium chloride is 5*10<-3>-15*10<-3> mol / L, and the concentration of the manganese sulfate is 6*10<-3>-16*10<-3> mol / L. The invention solves the problem that nutrients contained in the soybeans can not be sufficiently activated in the traditional soybean sprouting technology.

Description

technical field [0001] The present invention relates to a culture solution for increasing the peptide content in germinated soybean and its application method. Background technique [0002] Soybean is rich in protein, reasonable in amino acid composition and structure, and its nutritional value is close to that of milk and eggs. It is a high-quality vegetable protein. And soybeans are rich in physiologically active substances that have important health care functions for the human body. However, there are some anti-nutritional factors in soybeans, such as trypsin inhibitors and hemagglutinin in soybeans, which restrict the digestion and utilization of protein by the human body, and phytic acid substances restrict the utilization of mineral elements by the body. Therefore, the use of biotechnology to remove anti-nutritional factors in soybeans is conducive to improving the nutritional value of soybeans. [0003] Complete soybean seeds have vitality and can absorb water to g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 吴志坚顾振新张红茅晓慧
Owner 常州五星禾绿蔬菜食品有限公司
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