Culture solution increasing content of peptides contained in sprouted soybeans
A technology for germinating soybeans and culture solution, which is applied in fertilizer mixtures, fertilization devices, applications, etc., can solve the problems of insufficient stimulation of soybean nutrition, and achieve the effects of easy industrial production, easy operation, and scientific and reasonable formula.
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Embodiment 1
[0016] In the culture medium, the concentration of gibberellin was 55×10 -6 mol / L, the concentration of calcium chloride is 6×10 -3 The concentration of mol / L and manganese sulfate is 10×10 -3 mol / L.
[0017] Select mature, plump and undamaged soybeans, immerse them in a mass ratio of 1% sodium hypochlorite solution for disinfection for 30 minutes at a ratio of 1:5, and then rinse them with water; place the disinfected soybeans in a pH 5.2 citric acid-sodium citrate buffer solution at a ratio of 1:5 Soak in (CBS) at a temperature of 25°C for 6 hours, then transfer to the above-mentioned culture solution for infiltration and cultivation for 6 days, during which time the culture solution is sprayed on the germinated soybeans every 12 hours, the culture temperature is 15-29°C, and the culture environment is kept moist. . The culture medium used was taken out after 6 days of germination, and the peptide content in germinated soybeans was 102.47 mg·g -1 .
Embodiment 2
[0019] In the culture medium, the concentration of gibberellin is 72×10 -6 mol / L, the concentration of calcium chloride is 7.5×10 -3 The concentration of mol / L and manganese sulfate is 12×10 -3 mol / L.
[0020] Select mature, plump and undamaged soybeans, immerse them in a mass ratio of 1% sodium hypochlorite solution for disinfection for 30 minutes at a ratio of 1:5, and then rinse them with water; place the disinfected soybeans in a pH 5.2 citric acid-sodium citrate buffer solution at a ratio of 1:5 Soak in (CBS) at a temperature of 25°C for 6 hours, then transfer to the above-mentioned culture solution for infiltration and cultivation for 6 days, during which time the culture solution is sprayed on the germinated soybeans every 12 hours, the culture temperature is 15-29°C, and the culture environment is kept moist. . The culture medium used was taken out after 6 days of germination, and the peptide content in germinated soybeans was 122.49 mg·g -1 .
Embodiment 3
[0022] In the culture medium, the concentration of gibberellin was 45×10 -6 mol / L, the concentration of calcium chloride is 12×10 -3 The concentration of mol / L and manganese sulfate is 10×10 -3 mol / L.
[0023] Select mature, plump and undamaged soybeans, immerse them in a mass ratio of 1% sodium hypochlorite solution for disinfection for 30 minutes at a ratio of 1:5, and then rinse them with water; place the disinfected soybeans in a pH 5.2 citric acid-sodium citrate buffer solution at a ratio of 1:5 Soak in (CBS) at a temperature of 25°C for 6 hours, then transfer to the above-mentioned culture solution for infiltration and cultivation for 6 days, during which time the culture solution is sprayed on the germinated soybeans every 12 hours, the culture temperature is 15-29°C, and the culture environment is kept moist. . The culture medium used was taken out after 6 days of germination, and the peptide content in germinated soybeans was 142.13 mg·g -1 .
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