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Production, formulation, and uses of stable liquid harpin protein formulations

A protease inhibitor, protein technology, applied in the direction of herbicides and algicides, applications, biocides, etc., can solve the problems of limiting the use and versatility of harpin protein

Inactive Publication Date: 2011-08-10
PLANT HEALTH CARE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use and versatility of powdered harpin proteins is limited by the fact that suspensions of powdered harpin proteins in water have an effective lifetime of only 48-72 hours before significant degradation and inactivation occur

Method used

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  • Production, formulation, and uses of stable liquid harpin protein formulations
  • Production, formulation, and uses of stable liquid harpin protein formulations
  • Production, formulation, and uses of stable liquid harpin protein formulations

Examples

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Effect test

example 1

[0098] Example 1 - Containing Harpin αβ * Preparation of stable liquid compositions of and their efficacy in disease resistance tests

[0099] Recombinant E. coli was used to express harpin αβ (superharpin of SEQ ID NO: 1 ) under the control of a constitutive promoter. After fermentation, the suspension was diluted in 2 volumes of potassium phosphate buffer to adjust the pH of the suspension to 7.0.

[0100] The resulting solution was heat-treated at 95°C for 1 minute using a heat exchange system and then cooled to 45°C within 40 minutes.

[0101] Lysozyme was added to the solution after reaching about 38-42°C, mixed to a final concentration of 1 ppm, and allowed to react for 45 minutes. This facilitates the rupture of the cell wall of the bacteria, resulting in the formation of a crude harpin αβ extract.

[0102] The resulting crude extract was then centrifuged for 5 minutes to remove some cellular debris. The resulting supernatant was separated and subjected to further h...

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Abstract

The present invention relates to a method of making a stable liquid composition containing a harpin protein or polypeptide. Also disclosed is a composition comprising an aqueous carrier, a harpin protein or polypeptide, an effective amount of a biocidal agent, and optionally, an effective amount of one or both of a protease inhibitor and a non-ionic surfactant. The composition retains harpin activity for at least about 72 hours. The present invention also relates to a method of inducing a plant response by applying to a plant or a plant seed the composition of the present invention.

Description

[0001] This application claims priority to US Provisional Patent Application Serial No. 61 / 088,195, filed August 12, 2008, which is hereby incorporated by reference in its entirety. technical field [0002] The invention relates to the preparation, formulation and use of a stable liquid harpin protein preparation. Background technique [0003] Plants have evolved a complex set of biochemical pathways that recognize and respond to environmental cues including pathogen infection. There are two main types of interactions between pathogens and plants - compatible and incompatible. Disease usually occurs when the pathogen and the plant are compatible. If the pathogen and the plant are incompatible, the plant is usually resistant to that particular pathogen. In an incompatible interaction, plants limit the propagation of the pathogen by causing a small area of ​​localized necrosis or tissue death around the site of infection. This response in plants is defined as a hypersensiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01P3/00A01N63/02A01P5/00A01P7/00A01P19/00A01P21/00A01P1/00A01P13/00A01N25/02
CPCA01N63/02C07K14/27C07K14/195C07K14/21A01N25/22A01N63/10A01N2300/00A01N63/50A01N63/20A01N25/02A01N33/12A01N63/27
Inventor Z 魏
Owner PLANT HEALTH CARE INC
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