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Bacillussubtilis and application thereof in raising Apostichopus japonicus

A technology of Bacillus subtilis and Apostichopus japonicus, which is applied in application, medical preparations containing active ingredients, bacteria, etc., can solve the problems of ecological environment destruction of farmed animals, growth inhibition of farmed animals, inability to guarantee food safety, etc. Effects of aquaculture product quality, restoration of ecological environment, and prevention of pathogenic bacteria resistance

Inactive Publication Date: 2011-07-27
LIAONING OCEAN & FISHERIES SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is recognized that the disease prevention and control of farmed animals is controlled by drugs. Due to the use of antibiotics, the ecological environment of farmed animals is destroyed, the growth of farmed animals is inhibited, and food safety cannot be guaranteed. Therefore, the use of antibiotics is strictly restricted.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] In the planktonic larvae stage of sea cucumber seedlings, the method of using Bacillus subtilis (Sn) that can effectively inhibit pathogenic bacteria such as Vibrio and promote the growth of sea cucumber seedlings alone is: make the above-mentioned Bacillus subtilis Sn with a live bacteria content greater than 10 9 The cfu / ml bacterial solution was evenly sprinkled into the sea cucumber larvae cultivation pool, the growth of the sea cucumber larvae was measured and the number of Vibrio was detected with TCBS medium.

[0015] Trial Design, Management, Measurements and Results

[0016] The planktonic larvae of sea cucumbers that had just hatched were selected and randomly divided into two test groups, with three parallel samples in each group. They were blank group and Sn probiotic group respectively. The breeding pond is 0.4m 3 / tank, sea cucumber planktonic larvae bait according to marine yeast 2-3ml / m 3 Feeding, the feeding amount and other test conditions of each ...

Embodiment 2

[0023] Apply the Bacillus subtilis and the method of adding carrier involved in the present invention to the production of imitation japonicus japonicus seedlings, observe the growth conditions of the experimental group and the control group, and use TCBS medium to detect the number of vibrio. The above-mentioned Bacillus subtilis Sn is uniformly mixed with a carrier to prepare a live bacteria composition. The method is as follows: 3 parts of bacterial liquid are placed in a clean container, 1 part of calcium-based bentonite is slowly poured into the bacterial liquid, and stirred while pouring, so that the bentonite and the bacterial liquid are fully mixed.

[0024] Trial Design, Management, Measurements and Results

[0025] Select ginseng seedlings with a body length of 0.5-2cm and a body weight of 0.01-0.017g (30,000-50,000 heads / 500g), and randomly divide them into 2 test groups, each with 3 parallel samples. They are control group, Bacillus subtilis Sn and carrier group r...

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PUM

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Abstract

The invention relates to separation of Bacillussubtilis and application of the Bacillussubtilis in raising Apostichopus japonicus. The Bacillussubtilis can effectively inhibit vibriones and other pathogenic bacteria from breeding, and promote the growth of the Apostichopus japonicus. The strain is separated from gills of fished captured in the Dalian sea area, and is preserved by China General Microbiological Culture Collection Center with the collection number of CGMCC No.3498. The Bacillussubtilis can be widely applied to breeding and production links in the fishery industry, can degrade, convert and metabolize metabolic products in the breeding and production processes, creates good ecological environment, and improves, preserves and repairs the ecological environment of water resources to fulfill the aims of reducing self-pollution, reducing medicinal harm, reducing cost, lightening the pollution of the aquaculture to the whole ecological environment and improving the quality of cultured products.

Description

technical field [0001] The invention relates to the isolation of a strain of Bacillus subtilis and its use method in raising imitation sea cucumber seedlings. The strain can effectively inhibit the reproduction of pathogenic bacteria such as Vibrio in the seawater during the cultivation of aquatic organisms and can promote the growth of sea cucumber imitation sea cucumbers . Background technique [0002] At present, with the rapid development of high-density mariculture industry and the intensification of environmental pollution, the disease of farmed animals has become a major problem in the development of mariculture industry in my country and even the world. It is recognized that the disease prevention and control of farmed animals is controlled by drugs. Due to the use of antibiotics, the ecological environment of farmed animals is destroyed, the growth of farmed animals is inhibited, and food safety cannot be guaranteed. Therefore, the use of antibiotics is strictly lim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01K61/00A01N63/02A61K35/74A01P1/00A61P43/00C12R1/125A61K35/742
CPCY02A40/81
Inventor 苏浩刘卫东周遵春王志松王军鲍相勃
Owner LIAONING OCEAN & FISHERIES SCI RES INST
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