Bacillussubtilis and application thereof in raising Apostichopus japonicus
A technology of Bacillus subtilis and Apostichopus japonicus, which is applied in application, medical preparations containing active ingredients, bacteria, etc., can solve the problems of ecological environment destruction of farmed animals, growth inhibition of farmed animals, inability to guarantee food safety, etc. Effects of aquaculture product quality, restoration of ecological environment, and prevention of pathogenic bacteria resistance
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Embodiment 1
[0014] In the planktonic larvae stage of sea cucumber seedlings, the method of using Bacillus subtilis (Sn) that can effectively inhibit pathogenic bacteria such as Vibrio and promote the growth of sea cucumber seedlings alone is: make the above-mentioned Bacillus subtilis Sn with a live bacteria content greater than 10 9 The cfu / ml bacterial solution was evenly sprinkled into the sea cucumber larvae cultivation pool, the growth of the sea cucumber larvae was measured and the number of Vibrio was detected with TCBS medium.
[0015] Trial Design, Management, Measurements and Results
[0016] The planktonic larvae of sea cucumbers that had just hatched were selected and randomly divided into two test groups, with three parallel samples in each group. They were blank group and Sn probiotic group respectively. The breeding pond is 0.4m 3 / tank, sea cucumber planktonic larvae bait according to marine yeast 2-3ml / m 3 Feeding, the feeding amount and other test conditions of each ...
Embodiment 2
[0023] Apply the Bacillus subtilis and the method of adding carrier involved in the present invention to the production of imitation japonicus japonicus seedlings, observe the growth conditions of the experimental group and the control group, and use TCBS medium to detect the number of vibrio. The above-mentioned Bacillus subtilis Sn is uniformly mixed with a carrier to prepare a live bacteria composition. The method is as follows: 3 parts of bacterial liquid are placed in a clean container, 1 part of calcium-based bentonite is slowly poured into the bacterial liquid, and stirred while pouring, so that the bentonite and the bacterial liquid are fully mixed.
[0024] Trial Design, Management, Measurements and Results
[0025] Select ginseng seedlings with a body length of 0.5-2cm and a body weight of 0.01-0.017g (30,000-50,000 heads / 500g), and randomly divide them into 2 test groups, each with 3 parallel samples. They are control group, Bacillus subtilis Sn and carrier group r...
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